miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas

Maria Braoudaki; G I Lambrou; K Giannikou; S A Papadodima; A Lykoudi; K Stefanaki; G Sfakianos; A Kolialexi; F Tzortzatou-Stathopoulou; M Tzetis; S Kitsiou-Tzeli; E Kanavakis

doi:10.1007/s13277-016-4903-7

miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas

Maria Braoudaki, G I Lambrou, K Giannikou, S A Papadodima, A Lykoudi, K Stefanaki, G Sfakianos, A Kolialexi, F Tzortzatou-Stathopoulou, M Tzetis, S Kitsiou-Tzeli, E Kanavakis

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17 Citations (Scopus)

Abstract

In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma.

Original language	English
Pages (from-to)	9887-97
Number of pages	11
Journal	Tumor Biology
Volume	37
Issue number	7
Early online date	26 Jan 2016
DOIs	https://doi.org/10.1007/s13277-016-4903-7
Publication status	E-pub ahead of print - 26 Jan 2016

Keywords

Adolescent
Astrocytoma/genetics
Biomarkers, Tumor/genetics
Case-Control Studies
Child
Disease Progression
Ependymoma/genetics
Female
Follow-Up Studies
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Male
MicroRNAs/genetics
Neoplasm Staging
Prognosis
RNA, Messenger/genetics
ROC Curve
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction

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10.1007/s13277-016-4903-7

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Braoudaki, M., Lambrou, G. I., Giannikou, K., Papadodima, S. A., Lykoudi, A., Stefanaki, K., Sfakianos, G., Kolialexi, A., Tzortzatou-Stathopoulou, F., Tzetis, M., Kitsiou-Tzeli, S., & Kanavakis, E. (2016). miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas. Tumor Biology, 37(7), 9887-97. Advance online publication. https://doi.org/10.1007/s13277-016-4903-7

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title = "miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas",

abstract = "In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma. ",

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author = "Maria Braoudaki and Lambrou, {G I} and K Giannikou and Papadodima, {S A} and A Lykoudi and K Stefanaki and G Sfakianos and A Kolialexi and F Tzortzatou-Stathopoulou and M Tzetis and S Kitsiou-Tzeli and E Kanavakis",

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doi = "10.1007/s13277-016-4903-7",

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Braoudaki, M, Lambrou, GI, Giannikou, K, Papadodima, SA, Lykoudi, A, Stefanaki, K, Sfakianos, G, Kolialexi, A, Tzortzatou-Stathopoulou, F, Tzetis, M, Kitsiou-Tzeli, S & Kanavakis, E 2016, 'miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas', Tumor Biology, vol. 37, no. 7, pp. 9887-97. https://doi.org/10.1007/s13277-016-4903-7

TY - JOUR

T1 - miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas

AU - Braoudaki, Maria

AU - Lambrou, G I

AU - Giannikou, K

AU - Papadodima, S A

AU - Lykoudi, A

AU - Stefanaki, K

AU - Sfakianos, G

AU - Kolialexi, A

AU - Tzortzatou-Stathopoulou, F

AU - Tzetis, M

AU - Kitsiou-Tzeli, S

AU - Kanavakis, E

PY - 2016/1/26

Y1 - 2016/1/26

N2 - In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma.

AB - In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma.

KW - Adolescent

KW - Astrocytoma/genetics

KW - Biomarkers, Tumor/genetics

KW - Case-Control Studies

KW - Child

KW - Disease Progression

KW - Ependymoma/genetics

KW - Female

KW - Follow-Up Studies

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Humans

KW - Male

KW - MicroRNAs/genetics

KW - Neoplasm Staging

KW - Prognosis

KW - RNA, Messenger/genetics

KW - ROC Curve

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1007/s13277-016-4903-7

DO - 10.1007/s13277-016-4903-7

M3 - Article

C2 - 26813564

SN - 1010-4283

VL - 37

SP - 9887

EP - 9897

JO - Tumor Biology

JF - Tumor Biology

IS - 7

ER -

miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas

Abstract

Keywords

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