Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

Anthony J Atkin; Jason M Lynam; Benjamin E Moulton; Philip Sawle; Roberto Motterlini; Nicola M Boyle; Mary T Pryce; Ian J S Fairlamb

doi:10.1039/c0dt01809k

Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

Anthony J Atkin
, Jason M Lynam
, Benjamin E Moulton
, Philip Sawle
, Roberto Motterlini
, Nicola M Boyle
, Mary T Pryce
, Ian J S Fairlamb

Department of Clinical, Pharmaceutical and Biological Science

Research output: Contribution to journal › Article › peer-review

182 Citations (Scopus)

Abstract

The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.

Original language	English
Pages (from-to)	5755-61
Number of pages	7
Journal	Dalton Transactions
Volume	40
Issue number	21
DOIs	https://doi.org/10.1039/c0dt01809k
Publication status	Published - 7 Jun 2011

Keywords

Carbon Monoxide
Coordination Complexes
Hydrogen-Ion Concentration
Myoglobin
Organometallic Compounds
Spectrophotometry, Ultraviolet
Journal Article
Research Support, Non-U.S. Gov't

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10.1039/c0dt01809k

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title = "Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes",

abstract = "The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.",

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year = "2011",

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doi = "10.1039/c0dt01809k",

language = "English",

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AU - Lynam, Jason M

AU - Moulton, Benjamin E

AU - Sawle, Philip

AU - Motterlini, Roberto

AU - Boyle, Nicola M

AU - Pryce, Mary T

AU - Fairlamb, Ian J S

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AB - The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.

KW - Carbon Monoxide

KW - Coordination Complexes

KW - Hydrogen-Ion Concentration

KW - Myoglobin

KW - Organometallic Compounds

KW - Spectrophotometry, Ultraviolet

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1039/c0dt01809k

DO - 10.1039/c0dt01809k

M3 - Article

C2 - 21523300

SN - 1477-9226

VL - 40

SP - 5755

EP - 5761

JO - Dalton Transactions

JF - Dalton Transactions

IS - 21

ER -

Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

Abstract

Keywords

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