Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of Arabidopsis thaliana

Raquel Iglesias-Fernández; María del Carmen Rodríguez-Gacio; Cristina Barrero-Sicilia; Pilar Carbonero; Angel J. Matilla

doi:10.4161/psb/14126

Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of Arabidopsis thaliana

Raquel Iglesias-Fernández, María del Carmen Rodríguez-Gacio, Cristina Barrero-Sicilia, Pilar Carbonero, Angel J. Matilla

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The endo-β-mannanase (MAN) family is represented in the Arabidopsis genome by eight members, all with canonical signal peptides and only half of them being expressed in germinating seeds. The transcripts of these genes were localized in the radicle and micropylar endosperm (ME) before radicle protrusion and this expression disappears as soon as the endosperm is broken by the emerging radicle tip. However, only three of these MAN genes, AtMAN5, AtMAN7 and especially AtMAN6 influence the germination time (t) as assessed by the analysis of the corresponding knock-out lines. The data suggest a possible interaction between embryo and ME regarding the role of MAN during the Arabidopsis germination process.

Original language	English
Journal	Plant Signaling and Behavior
Volume	6
Issue number	1
DOIs	https://doi.org/10.4161/psb/14126
Publication status	Published - Jan 2011

Keywords

Arabidopsis
Endo-β-mannanases
Germination
In situ hybridization
Micropylar endosperm
T-DNA insertion mutants

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title = "Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of Arabidopsis thaliana",

abstract = "The endo-β-mannanase (MAN) family is represented in the Arabidopsis genome by eight members, all with canonical signal peptides and only half of them being expressed in germinating seeds. The transcripts of these genes were localized in the radicle and micropylar endosperm (ME) before radicle protrusion and this expression disappears as soon as the endosperm is broken by the emerging radicle tip. However, only three of these MAN genes, AtMAN5, AtMAN7 and especially AtMAN6 influence the germination time (t) as assessed by the analysis of the corresponding knock-out lines. The data suggest a possible interaction between embryo and ME regarding the role of MAN during the Arabidopsis germination process.",

keywords = "Arabidopsis, Endo-β-mannanases, Germination, In situ hybridization, Micropylar endosperm, T-DNA insertion mutants",

author = "Raquel Iglesias-Fern{\'a}ndez and Rodr{\'i}guez-Gacio, {Mar{\'i}a del Carmen} and Cristina Barrero-Sicilia and Pilar Carbonero and Matilla, {Angel J.}",

year = "2011",

month = jan,

doi = "10.4161/psb/14126",

language = "English",

volume = "6",

journal = "Plant Signaling and Behavior",

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publisher = "Landes Bioscience",

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Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of Arabidopsis thaliana. / Iglesias-Fernández, Raquel; Rodríguez-Gacio, María del Carmen; Barrero-Sicilia, Cristina et al.
In: Plant Signaling and Behavior, Vol. 6, No. 1, 01.2011.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of Arabidopsis thaliana

AU - Iglesias-Fernández, Raquel

AU - Rodríguez-Gacio, María del Carmen

AU - Barrero-Sicilia, Cristina

AU - Carbonero, Pilar

AU - Matilla, Angel J.

PY - 2011/1

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AB - The endo-β-mannanase (MAN) family is represented in the Arabidopsis genome by eight members, all with canonical signal peptides and only half of them being expressed in germinating seeds. The transcripts of these genes were localized in the radicle and micropylar endosperm (ME) before radicle protrusion and this expression disappears as soon as the endosperm is broken by the emerging radicle tip. However, only three of these MAN genes, AtMAN5, AtMAN7 and especially AtMAN6 influence the germination time (t) as assessed by the analysis of the corresponding knock-out lines. The data suggest a possible interaction between embryo and ME regarding the role of MAN during the Arabidopsis germination process.

KW - Arabidopsis

KW - Endo-β-mannanases

KW - Germination

KW - In situ hybridization

KW - Micropylar endosperm

KW - T-DNA insertion mutants

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DO - 10.4161/psb/14126

M3 - Article

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AN - SCOPUS:79956219858

SN - 1559-2316

VL - 6

JO - Plant Signaling and Behavior

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IS - 1

ER -

Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of Arabidopsis thaliana

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Keywords

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