Abstract
The incomplete sequences of two large, 10-12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode fusion proteins which are apparently expressed by a translational frameshifting mechanism. The C-terminal region of both predicted proteins was 21% identical and contained the eight motifs conserved in RNA-dependent RNA polymerases of dsRNA mycoviruses and had highest similarity with members of the family Totiviridae, but possibly do not form virions. The remainder of the N-terminal protein sequences predicted from the PgV-TW2 dsRNA1 and dsRNA2 sequences and the 3'-terminal nucleotide sequences of both dsRNAs had no homology with one another or any sequence in the database suggesting that individually both may be members of novel families of mycoviruses.
Original language | English |
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Pages (from-to) | 132-6 |
Number of pages | 5 |
Journal | Virus Genes |
Volume | 39 |
Issue number | 1 |
DOIs | |
Publication status | Published - Aug 2009 |
Keywords
- Phylogeny
- Viral Proteins
- RNA, Double-Stranded
- Basidiomycota
- Totiviridae
- Molecular Sequence Data
- Amino Acid Sequence
- Sequence Homology, Amino Acid
- Sequence Analysis, DNA
- RNA Replicase