TY - JOUR
T1 - Molecular characterization of a polymycovirus in Leptosphaeria biglobosa
AU - Samir, Nesma M.
AU - Locke-Gotel, Jacob
AU - Urayama, Syun-Ichi
AU - El-Morsi, Adel A.
AU - El-Sherbeny, Ghada A.
AU - Huang, Yongju
AU - Fitt, Bruce D. L.
AU - Moriyama, Hiromitsu
AU - Coutts, Robert H. A.
AU - Kotta-Loizou, Ioly
N1 - © 2025 The Author(s). This is an open access article distributed under the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/
PY - 2025/4/1
Y1 - 2025/4/1
N2 - Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.
AB - Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.
KW - Phylogeny
KW - Fungal Viruses/genetics
KW - Genome, Viral/genetics
KW - Brassica napus/microbiology
KW - Open Reading Frames
KW - Plant Diseases/microbiology
KW - RNA, Double-Stranded/genetics
KW - RNA, Viral/genetics
KW - Leptosphaeria/genetics
KW - Viral Proteins/genetics
KW - RNA-Dependent RNA Polymerase/genetics
KW - Double Stranded RNA Viruses/genetics
KW - Ascomycota/virology
UR - http://www.scopus.com/inward/record.url?scp=86000684487&partnerID=8YFLogxK
U2 - 10.1007/s00705-025-06253-1
DO - 10.1007/s00705-025-06253-1
M3 - Article
C2 - 40050447
SN - 0304-8608
VL - 170
SP - 1
EP - 5
JO - Archives of Virology
JF - Archives of Virology
IS - 4
M1 - 66
ER -