Molecular characterization of a polymycovirus in Leptosphaeria biglobosa

Nesma M. Samir; Jacob Locke-Gotel; Syun-Ichi Urayama; Adel A. El-Morsi; Ghada A. El-Sherbeny; Yongju Huang; Bruce D. L. Fitt; Hiromitsu Moriyama; Robert H. A. Coutts; Ioly Kotta-Loizou

doi:10.1007/s00705-025-06253-1

Molecular characterization of a polymycovirus in Leptosphaeria biglobosa

Nesma M. Samir, Jacob Locke-Gotel, Syun-Ichi Urayama, Adel A. El-Morsi, Ghada A. El-Sherbeny, Yongju Huang, Bruce D. L. Fitt, Hiromitsu Moriyama, Robert H. A. Coutts, Ioly Kotta-Loizou

Research output: Contribution to journal › Article › peer-review

Abstract

Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.

Original language	English
Article number	66
Pages (from-to)	1-5
Number of pages	5
Journal	Archives of Virology
Volume	170
Issue number	4
Early online date	6 Mar 2025
DOIs	https://doi.org/10.1007/s00705-025-06253-1
Publication status	E-pub ahead of print - 6 Mar 2025

Keywords

Phylogeny
Fungal Viruses/genetics
Genome, Viral/genetics
Brassica napus/microbiology
Open Reading Frames
Plant Diseases/microbiology
RNA, Double-Stranded/genetics
RNA, Viral/genetics
Leptosphaeria/genetics
Viral Proteins/genetics
RNA-Dependent RNA Polymerase/genetics
Double Stranded RNA Viruses/genetics
Ascomycota/virology

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© 2025 The Author(s). This is an open access article distributed under the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/
Final published version, 1.6 MBLicence: CC BY

Cite this

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title = "Molecular characterization of a polymycovirus in Leptosphaeria biglobosa",

abstract = "Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named {"}Leptosphaeria biglobosa polymycovirus 1{"}, should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.",

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author = "Samir, {Nesma M.} and Jacob Locke-Gotel and Syun-Ichi Urayama and El-Morsi, {Adel A.} and El-Sherbeny, {Ghada A.} and Yongju Huang and Fitt, {Bruce D. L.} and Hiromitsu Moriyama and Coutts, {Robert H. A.} and Ioly Kotta-Loizou",

note = "{\textcopyright} 2025 The Author(s). This is an open access article distributed under the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/ ",

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month = mar,

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doi = "10.1007/s00705-025-06253-1",

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T1 - Molecular characterization of a polymycovirus in Leptosphaeria biglobosa

AU - Samir, Nesma M.

AU - Locke-Gotel, Jacob

AU - Urayama, Syun-Ichi

AU - El-Morsi, Adel A.

AU - El-Sherbeny, Ghada A.

AU - Huang, Yongju

AU - Fitt, Bruce D. L.

AU - Moriyama, Hiromitsu

AU - Coutts, Robert H. A.

AU - Kotta-Loizou, Ioly

PY - 2025/3/6

Y1 - 2025/3/6

N2 - Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.

AB - Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.

KW - Phylogeny

KW - Fungal Viruses/genetics

KW - Genome, Viral/genetics

KW - Brassica napus/microbiology

KW - Open Reading Frames

KW - Plant Diseases/microbiology

KW - RNA, Double-Stranded/genetics

KW - RNA, Viral/genetics

KW - Leptosphaeria/genetics

KW - Viral Proteins/genetics

KW - RNA-Dependent RNA Polymerase/genetics

KW - Double Stranded RNA Viruses/genetics

KW - Ascomycota/virology

U2 - 10.1007/s00705-025-06253-1

DO - 10.1007/s00705-025-06253-1

M3 - Article

C2 - 40050447

SN - 0304-8608

VL - 170

SP - 1

EP - 5

JO - Archives of Virology

JF - Archives of Virology

IS - 4

M1 - 66

ER -

Molecular characterization of a polymycovirus in Leptosphaeria biglobosa

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