TY - JOUR
T1 - Molecular epidemiology of predominant and emerging Clostridioides difficile ribotypes
AU - Adrian Martinez-Melendez, Adrian
AU - Morfin-Otero, Rayo
AU - Villarreal-Trevino, Licet
AU - Baines, Simon
AU - Camacho-Ortiz, Adrian
AU - Garza-Gonzalez, Elvira
N1 - © 2020 Elsevier B.V. All rights reserved.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - There has been an increase in the incidence and severity of Clostridioides difficile infection (CDI) worldwide, and strategies to control, monitor, and diminish the associated morbidity and mortality have been developed. Several typing methods have been used for typing of isolates and studying the epidemiology of CDI; serotyping was the first typing method, but then was replaced by pulsed-field gel electrophoresis (PFGE). PCR ribotyping is now the gold standard method; however, multi locus sequence typing (MLST) schemes have been developed. New sequencing technologies have allowed comparing whole bacterial genomes to address genetic relatedness with a high level of resolution and discriminatory power to distinguish between closely related strains. Here, we review the most frequent C. difficile ribotypes reported worldwide, with a focus on their epidemiology and genetic characteristics.
AB - There has been an increase in the incidence and severity of Clostridioides difficile infection (CDI) worldwide, and strategies to control, monitor, and diminish the associated morbidity and mortality have been developed. Several typing methods have been used for typing of isolates and studying the epidemiology of CDI; serotyping was the first typing method, but then was replaced by pulsed-field gel electrophoresis (PFGE). PCR ribotyping is now the gold standard method; however, multi locus sequence typing (MLST) schemes have been developed. New sequencing technologies have allowed comparing whole bacterial genomes to address genetic relatedness with a high level of resolution and discriminatory power to distinguish between closely related strains. Here, we review the most frequent C. difficile ribotypes reported worldwide, with a focus on their epidemiology and genetic characteristics.
U2 - 10.1016/j.mimet.2020.105974
DO - 10.1016/j.mimet.2020.105974
M3 - Article
SN - 1872-8359
VL - 175
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
M1 - 105974
ER -