Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate

J. Gaughran; N. Dimov; E. Clancy; T. Barry; T. J. Smith; J. Ducree

doi:10.1109/Transducers.2013.6626763

Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate

J. Gaughran, N. Dimov, E. Clancy, T. Barry, T. J. Smith, J. Ducree

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	305-308
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6626763
Publication status	Published - 2013
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13

Keywords

centrifugal microfluidics
solvent dependent routing
solvent triggered valving
Total RNA extraction

Access to Document

10.1109/Transducers.2013.6626763

Cite this

Gaughran, J., Dimov, N., Clancy, E., Barry, T., Smith, T. J., & Ducree, J. (2013). Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 305-308). Article 6626763 https://doi.org/10.1109/Transducers.2013.6626763

@inproceedings{f4efb709cfa04e77a44d3302d5febe57,

title = "Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate",

abstract = "We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.",

keywords = "centrifugal microfluidics, solvent dependent routing, solvent triggered valving, Total RNA extraction",

author = "J. Gaughran and N. Dimov and E. Clancy and T. Barry and Smith, \{T. J.\} and J. Ducree",

year = "2013",

doi = "10.1109/Transducers.2013.6626763",

language = "English",

isbn = "9781467359818",

pages = "305--308",

booktitle = "2013 Transducers and Eurosensors XXVII",

note = "2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 ; Conference date: 16-06-2013 Through 20-06-2013",

}

Gaughran, J, Dimov, N, Clancy, E, Barry, T, Smith, TJ & Ducree, J 2013, Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6626763, pp. 305-308, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6626763

Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate. / Gaughran, J.; Dimov, N.; Clancy, E. et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 305-308 6626763.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate

AU - Gaughran, J.

AU - Dimov, N.

AU - Clancy, E.

AU - Barry, T.

AU - Smith, T. J.

AU - Ducree, J.

PY - 2013

Y1 - 2013

N2 - We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.

AB - We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.

KW - centrifugal microfluidics

KW - solvent dependent routing

KW - solvent triggered valving

KW - Total RNA extraction

UR - https://www.scopus.com/pages/publications/84891704194

U2 - 10.1109/Transducers.2013.6626763

DO - 10.1109/Transducers.2013.6626763

M3 - Conference contribution

AN - SCOPUS:84891704194

SN - 9781467359818

SP - 305

EP - 308

BT - 2013 Transducers and Eurosensors XXVII

T2 - 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Y2 - 16 June 2013 through 20 June 2013

ER -

Gaughran J, Dimov N, Clancy E, Barry T, Smith TJ, Ducree J. Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 305-308. 6626763 doi: 10.1109/Transducers.2013.6626763

Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this