This study was to investigate the neurotoxicity of multi-walled carbon nanotube (MWCNT) by measuring neuronal excitability in rat hippocampal neurons and exploring the underlying mechanism. Whole cell patch-clamp technique was used. Action potential properties and the pattern of repetitive firing rate were assessed. Our data showed that spike half-width and repetitive firing rate were significantly increased in a concentration-dependent manner. Furthermore, voltage-activated potassium currents were recorded. It was found that MWCNT produced a concentration-dependent inhibition in amplitudes of IA and IK. In addition, MWCNT had effect on the activation kinetics of IA and IK with Vh being shifted to the negative potential at high concentration, while IA inactivation curve was considerably shifted to the hyperpolarize potential with Vh being increased. However, no effect was found on the recovery from inactivation of IA. The results suggest that MWCNT increases the excitability of hippocampal CA1 neurons by inhibiting voltage-gated potassium current.
- Transient outward potassium current (I-A)
- Delayed rectifier potassium current (I-K)
- Evoked action potential
- TEMPORAL-LOBE EPILEPSY
- PYRAMIDAL NEURONS