Multiple forms of bovine pepsinogen: isolation and identification in serum from calves with ostertagiasis

P. D. Eckersall; J. Macaskill; Quintin McKellar; K. L. Bryce

Multiple forms of bovine pepsinogen: isolation and identification in serum from calves with ostertagiasis

P. D. Eckersall, J. Macaskill, Quintin McKellar, K. L. Bryce

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8 Citations (Scopus)

Abstract

By using fast protein liquid chromatography (FPLC) a new form of bovine pepsinogen has been identified. Compared with the previously isolated pepsinogen (PG I) the new pepsinogen (PG II) is less mobile on agarose electrophoresis, is eluted earlier from an anion-exchange column and is 30 times less abundant. Agarose electrophoresis identified PG I in the serum of calves given third stage Ostertagia ostertagi larvae, while FPLC identified both PG I and PG II in such serum.

Original language	English
Pages (from-to)	279-83
Number of pages	5
Journal	Research in Veterinary Science
Volume	43
Issue number	3
Publication status	Published - Nov 1987

Cite this

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title = "Multiple forms of bovine pepsinogen: isolation and identification in serum from calves with ostertagiasis",

abstract = "By using fast protein liquid chromatography (FPLC) a new form of bovine pepsinogen has been identified. Compared with the previously isolated pepsinogen (PG I) the new pepsinogen (PG II) is less mobile on agarose electrophoresis, is eluted earlier from an anion-exchange column and is 30 times less abundant. Agarose electrophoresis identified PG I in the serum of calves given third stage Ostertagia ostertagi larvae, while FPLC identified both PG I and PG II in such serum.",

author = "Eckersall, {P. D.} and J. Macaskill and Quintin McKellar and Bryce, {K. L.}",

year = "1987",

month = nov,

language = "English",

volume = "43",

pages = "279--83",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - Multiple forms of bovine pepsinogen

T2 - isolation and identification in serum from calves with ostertagiasis

AU - Eckersall, P. D.

AU - Macaskill, J.

AU - McKellar, Quintin

AU - Bryce, K. L.

PY - 1987/11

Y1 - 1987/11

N2 - By using fast protein liquid chromatography (FPLC) a new form of bovine pepsinogen has been identified. Compared with the previously isolated pepsinogen (PG I) the new pepsinogen (PG II) is less mobile on agarose electrophoresis, is eluted earlier from an anion-exchange column and is 30 times less abundant. Agarose electrophoresis identified PG I in the serum of calves given third stage Ostertagia ostertagi larvae, while FPLC identified both PG I and PG II in such serum.

AB - By using fast protein liquid chromatography (FPLC) a new form of bovine pepsinogen has been identified. Compared with the previously isolated pepsinogen (PG I) the new pepsinogen (PG II) is less mobile on agarose electrophoresis, is eluted earlier from an anion-exchange column and is 30 times less abundant. Agarose electrophoresis identified PG I in the serum of calves given third stage Ostertagia ostertagi larvae, while FPLC identified both PG I and PG II in such serum.

M3 - Article

C2 - 3444973

SN - 0034-5288

VL - 43

SP - 279

EP - 283

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 3

ER -

Multiple forms of bovine pepsinogen: isolation and identification in serum from calves with ostertagiasis

Abstract

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