Mutation analysis of cis-elements in the 3'- and 5'-untranslated regions of satellite tobacco necrosis virus strain C RNA

D.H. Bringloe, Robert H.A. Coutts, C.W.A. Pleij

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

The putative, 3'-terminal stem-loop structure in satellite tobacco necrosis virus strain C (STNV-C) RNA constitutes an essential cis-acting structure for the promotion of negative-strand RNA synthesis and a single- stranded tail is also important. The putative, 5'-terminal stem-loop structure in STNV-C RNA is not essential for productive, plus-strand RNA accumulation but is required for optimal accumulation. Residues 2 and 3 are the minimal cis-acting sequences required for RNA synthesis. The RNA of chimeric mutants, which exchanged 3'- and 5'-untranslated regions between STNV-C and helper tobacco necrosis virus strain D RNAs, accumulated in protoplasts, implying similar replication mechanisms for both RNAs.
Original languageEnglish
Pages (from-to)76-84
Number of pages9
JournalJournal of General Virology
Volume264
Issue number1
DOIs
Publication statusPublished - 10 Nov 1999

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