TY - JOUR
T1 - Negatively supercoiled DNA from plants infected with a single-stranded DNA virus
AU - Sunter, G.
AU - Coutts, Robert H.A.
AU - Buck, K.W.
PY - 1984/2/14
Y1 - 1984/2/14
N2 - A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme
AB - A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme
UR - http://www.scopus.com/inward/record.url?scp=0021762029&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(84)91458-X
DO - 10.1016/0006-291X(84)91458-X
M3 - Article
AN - SCOPUS:0021762029
SN - 0006-291X
VL - 118
SP - 747
EP - 752
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -