Negatively supercoiled DNA from plants infected with a single-stranded DNA virus

G. Sunter; Robert H.A. Coutts; K.W. Buck

doi:10.1016/0006-291X(84)91458-X

Negatively supercoiled DNA from plants infected with a single-stranded DNA virus

G. Sunter
, Robert H.A. Coutts
, K.W. Buck

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme

Original language	English
Pages (from-to)	747-752
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	118
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(84)91458-X
Publication status	Published - 14 Feb 1984

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10.1016/0006-291X(84)91458-X

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title = "Negatively supercoiled DNA from plants infected with a single-stranded DNA virus",

abstract = "A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme",

author = "G. Sunter and Coutts, \{Robert H.A.\} and K.W. Buck",

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AU - Sunter, G.

AU - Coutts, Robert H.A.

AU - Buck, K.W.

PY - 1984/2/14

Y1 - 1984/2/14

N2 - A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme

AB - A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of - 0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme

UR - https://www.scopus.com/pages/publications/0021762029

U2 - 10.1016/0006-291X(84)91458-X

DO - 10.1016/0006-291X(84)91458-X

M3 - Article

AN - SCOPUS:0021762029

SN - 0006-291X

VL - 118

SP - 747

EP - 752

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Negatively supercoiled DNA from plants infected with a single-stranded DNA virus

Abstract

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