New methods for the detection of insecticide resistant Myzus persicae in the UK suction trap network

James A. Anstead, Martin S. Williamson, Ian Denholm

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


1 Myzus persicae is a highly polyphagous pest of U.K. agriculture. It presents particular control difficulties because it has developed resistance to several insecticide classes.
2 For almost 20 years, M. persicae collected in the U.K. suction trap network have been analysed for insecticide resistance and the data disseminated to growers via a resistance bulletin. These data are generated by the biochemical analysis of individuals for two major resistance phenotypes: (i) elevated carboxylesterase and (ii) modified acetylcholinesterase (MACE).
3 The development of new polymerase chain reaction (PCR)-based technologies using fluorescently labelled probes has allowed other resistance mechanisms, such as knockdown resistance to pyrethroids (kdr/super-kdr), to be detected and has greatly increased the speed and accuracy of resistance monitoring. Unfortunately, these newer PCR-based assays are incompatible with the older biochemical assays.
4 The present study describes the development and testing of new compatible methods for detecting elevated carboxylesterases and MACE for use on M. persicae caught in the field or suction traps.
5 These new tests have significant advantages over present methodologies by allowing individual aphids to be tested for three resistance mechanisms quickly and accurately on a single platform.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalAgricultural and Forest Entomology
Issue number3
Early online date28 Jun 2008
Publication statusPublished - 10 Jul 2008


  • GENE
  • carboxylesterase
  • MACE
  • insecticide resistance
  • Myzus persicae


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