P-glycoprotein does not play a significant role in 3H-digoxin transport in Calu-3 human bronchial epithelial cell monolayers

Victoria Hutter; Constanze Hilgendorf; Vanessa Zann; Anne Cooper; David Pritchard; Cynthia Bosquillon

P-glycoprotein does not play a significant role in 3H-digoxin transport in Calu-3 human bronchial epithelial cell monolayers

Victoria Hutter, Constanze Hilgendorf, Vanessa Zann, Anne Cooper, David Pritchard, Cynthia Bosquillon

Research output: Contribution to conference › Abstract

Abstract

The expression and functionality of
P-glycoprotein (P-gp) was assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Net secretory transport of the established P-glycoprotein substrate 3H-digoxin was abolished at 4°C but was only marginally affected by the metabolic inhibitor sodium azide and unaffected by the specific P-gp antibody inhibitor UIC2. Results suggest that P-gp does not play a major role in the trafficking of 3H-digoxin in Calu-3 cells.

Original language	English
Publication status	Published - 2011
Event	UKPharmSci 2011 - Nottingham, United Kingdom Duration: 31 Aug 2011 → 2 Sept 2011

Conference

Conference	UKPharmSci 2011
Country/Territory	United Kingdom
City	Nottingham
Period	31/08/11 → 2/09/11

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@conference{a351185ab95c47c793f386522b3e38a1,

title = "P-glycoprotein does not play a significant role in 3H-digoxin transport in Calu-3 human bronchial epithelial cell monolayers",

abstract = "The expression and functionality of P-glycoprotein (P-gp) was assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Net secretory transport of the established P-glycoprotein substrate 3H-digoxin was abolished at 4°C but was only marginally affected by the metabolic inhibitor sodium azide and unaffected by the specific P-gp antibody inhibitor UIC2. Results suggest that P-gp does not play a major role in the trafficking of 3H-digoxin in Calu-3 cells. ",

author = "Victoria Hutter and Constanze Hilgendorf and Vanessa Zann and Anne Cooper and David Pritchard and Cynthia Bosquillon",

year = "2011",

language = "English",

note = "UKPharmSci 2011 ; Conference date: 31-08-2011 Through 02-09-2011",

}

TY - CONF

T1 - P-glycoprotein does not play a significant role in 3H-digoxin transport in Calu-3 human bronchial epithelial cell monolayers

AU - Hutter, Victoria

AU - Hilgendorf, Constanze

AU - Zann, Vanessa

AU - Cooper, Anne

AU - Pritchard, David

AU - Bosquillon, Cynthia

PY - 2011

Y1 - 2011

N2 - The expression and functionality of P-glycoprotein (P-gp) was assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Net secretory transport of the established P-glycoprotein substrate 3H-digoxin was abolished at 4°C but was only marginally affected by the metabolic inhibitor sodium azide and unaffected by the specific P-gp antibody inhibitor UIC2. Results suggest that P-gp does not play a major role in the trafficking of 3H-digoxin in Calu-3 cells.

AB - The expression and functionality of P-glycoprotein (P-gp) was assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Net secretory transport of the established P-glycoprotein substrate 3H-digoxin was abolished at 4°C but was only marginally affected by the metabolic inhibitor sodium azide and unaffected by the specific P-gp antibody inhibitor UIC2. Results suggest that P-gp does not play a major role in the trafficking of 3H-digoxin in Calu-3 cells.

M3 - Abstract

T2 - UKPharmSci 2011

Y2 - 31 August 2011 through 2 September 2011

ER -

P-glycoprotein does not play a significant role in 3H-digoxin transport in Calu-3 human bronchial epithelial cell monolayers

Abstract

Conference

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