TY - JOUR
T1 - Pheromone synthesis in a biomicroreactor coated with anti-adsorption polyelectrolyte multilayer
AU - Dimov, Nikolay
AU - Muñoz, Lourdes
AU - Carot-Sans, Gerard
AU - Verhoeven, Michel L.P.M.
AU - Bula, Wojciech P.
AU - Kocer, Gülistan
AU - Guerrero, Angel
AU - Gardeniers, Han J.G.E.
PY - 2011/9/20
Y1 - 2011/9/20
N2 - To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His 6-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).
AB - To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His 6-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).
UR - http://www.scopus.com/inward/record.url?scp=80053460759&partnerID=8YFLogxK
U2 - 10.1063/1.3608138
DO - 10.1063/1.3608138
M3 - Article
AN - SCOPUS:80053460759
SN - 1932-1058
VL - 5
JO - Biomicrofluidics
JF - Biomicrofluidics
IS - 3
M1 - 034102
ER -