TY - JOUR
T1 - Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides
AU - Breda, Caroline Alves
AU - Gasperini, Alessandra Marcon
AU - Garcia, Vera Lucia
AU - Monteiro, Karin Maia
AU - Bataglion, Giovana Anceski
AU - Eberlin, Marcos Nogueira
AU - Duarte, Marta Cristina Teixeira
N1 - Publisher Copyright:
© 2016, The Author(s).
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Abstract: The increasing demand for safe food without preservatives or pesticides residues has encouraged several studies on natural products with antifungal activity and low toxicity. In this study, ethanolic extracts from leaves and fruit residues (peel and seeds) of three Brazilian savanna species (Acrocomia aculeata, Campomanesia adamantium and Caryocar brasiliense) were evaluated against phytopathogenic fungi. Additionally, the most active extract was chemically characterized by ESI-MS and its oral acute toxicity was evaluated. Extracts from C. brasiliense (pequi) peel and leaves were active against Alternaria alternata, Alternaria solani and Venturia pirina with minimal inhibitory concentrations between 350 and 1000 µg/mL. When incorporated in solid media, these extracts extended the lag phase of A. alternata and A. solani and reduced the growth rate of A. solani. Pequi peel extract showed better antifungal activity and their ESI-MS analysis revealed the presence of substances widely reported as antifungal such as gallic acid, quinic acid, ellagic acid, glucogalin and corilagin. The oral acute toxicity was relatively low, being considered safe for use as a potential natural fungicide. Graphical Abstract: [Figure not available: see fulltext.].
AB - Abstract: The increasing demand for safe food without preservatives or pesticides residues has encouraged several studies on natural products with antifungal activity and low toxicity. In this study, ethanolic extracts from leaves and fruit residues (peel and seeds) of three Brazilian savanna species (Acrocomia aculeata, Campomanesia adamantium and Caryocar brasiliense) were evaluated against phytopathogenic fungi. Additionally, the most active extract was chemically characterized by ESI-MS and its oral acute toxicity was evaluated. Extracts from C. brasiliense (pequi) peel and leaves were active against Alternaria alternata, Alternaria solani and Venturia pirina with minimal inhibitory concentrations between 350 and 1000 µg/mL. When incorporated in solid media, these extracts extended the lag phase of A. alternata and A. solani and reduced the growth rate of A. solani. Pequi peel extract showed better antifungal activity and their ESI-MS analysis revealed the presence of substances widely reported as antifungal such as gallic acid, quinic acid, ellagic acid, glucogalin and corilagin. The oral acute toxicity was relatively low, being considered safe for use as a potential natural fungicide. Graphical Abstract: [Figure not available: see fulltext.].
KW - Brazilian savanna fruits
KW - Natural fungicides
KW - Phytopathogens
KW - Residues
UR - http://www.scopus.com/inward/record.url?scp=85035105144&partnerID=8YFLogxK
U2 - 10.1007/s13659-016-0101-y
DO - 10.1007/s13659-016-0101-y
M3 - Article
AN - SCOPUS:85035105144
SN - 2192-2209
VL - 6
SP - 195
EP - 204
JO - Natural Products and Bioprospecting
JF - Natural Products and Bioprospecting
IS - 4
ER -