Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase

P.  Avila; F. Delacruz; Elaine Ward; J. Grinsted

doi:10.1007/BF00332761

Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase

P. Avila, F. Delacruz, Elaine Ward, J. Grinsted

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29 Citations (Scopus)

Abstract

In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition

Original language	English
Pages (from-to)	288-293
Number of pages	6
Journal	Molecular and General Genetics
Volume	195
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00332761
Publication status	Published - Jun 1984

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title = "Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase",

abstract = "In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition",

author = "P. Avila and F. Delacruz and Elaine Ward and J. Grinsted",

year = "1984",

month = jun,

doi = "10.1007/BF00332761",

language = "English",

volume = "195",

pages = "288--293",

journal = "Molecular and General Genetics",

issn = "0026-8925",

publisher = "Springer Nature ",

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T1 - Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase

AU - Avila, P.

AU - Delacruz, F.

AU - Ward, Elaine

AU - Grinsted, J.

PY - 1984/6

Y1 - 1984/6

N2 - In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition

AB - In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition

U2 - 10.1007/BF00332761

DO - 10.1007/BF00332761

M3 - Article

SN - 0026-8925

VL - 195

SP - 288

EP - 293

JO - Molecular and General Genetics

JF - Molecular and General Genetics

IS - 1-2

ER -

Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase

Abstract

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