Biologically active acetylcholinesterase from Drosophila melanogaster was constitutively expressed from a high copy number plasmid in Saccharomyces cerevisiae. Cellular metabolism and plasmid copy number were unaffected by expression. Based on comparative PMSF inhibition, the Kcat value (6430 mol s-1) was commensurate with human and Torpedo acetylcholinesterases. A 1.7 knt truncated mRNA transcript was observed on Northern blot analysis.

Original languageEnglish
Pages (from-to)417-422
Number of pages6
JournalBiotechnology Letters
Issue number6
Publication statusPublished - 1 Mar 2001


  • Acetylcholinesterase
  • Drosophila melanogaster
  • Saccharomyces cerevisiae


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