Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

Dan Stratton; Sigrun Lange; Jameel M Inal

doi:10.1016/j.bbrc.2012.12.012

Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

Dan Stratton, Sigrun Lange, Jameel M Inal

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10 Hz, 6V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release.

Original language	English
Pages (from-to)	470-5
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	430
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2012.12.012
Publication status	Published - 11 Jan 2013

Keywords

Apoptosis
Calcium
Cell Line, Tumor
Cell Membrane
Cell Survival
Chelating Agents
Complement Membrane Attack Complex
Egtazic Acid
Exosomes
Humans
Magnetic Fields
Organelles
Porosity
Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.bbrc.2012.12.012

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title = "Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells",

abstract = "Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10 Hz, 6V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release.",

keywords = "Apoptosis, Calcium, Cell Line, Tumor, Cell Membrane, Cell Survival, Chelating Agents, Complement Membrane Attack Complex, Egtazic Acid, Exosomes, Humans, Magnetic Fields, Organelles, Porosity, Journal Article, Research Support, Non-U.S. Gov't",

author = "Dan Stratton and Sigrun Lange and Inal, {Jameel M}",

year = "2013",

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doi = "10.1016/j.bbrc.2012.12.012",

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T1 - Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

AU - Stratton, Dan

AU - Lange, Sigrun

AU - Inal, Jameel M

PY - 2013/1/11

Y1 - 2013/1/11

N2 - Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10 Hz, 6V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release.

AB - Microvesicles are released from cell surfaces constitutively during early apoptosis or upon activation with various stimuli including sublytic membrane attack complex (MAC). This study shows that an alternating current, pulsed, extremely low-frequency electromagnetic field (0.3 μT at 10 Hz, 6V AC) induced transient plasma membrane damage that allowed calcium influx. This in turn caused a release of stimulated microvesicles (sMV). When extracellular calcium was chelated with EGTA, sMV biogenesis initiated by ELFMF was markedly reduced and the reduction was less than when the stimulation was the deposition of sublytic MAC. This suggested that pulsed ELFMF resulted in transcellular membrane pores causing organelles to leak additional calcium into the cytoplasm (which EGTA would not chelate) which itself can lead to sMV release.

KW - Apoptosis

KW - Calcium

KW - Cell Line, Tumor

KW - Cell Membrane

KW - Cell Survival

KW - Chelating Agents

KW - Complement Membrane Attack Complex

KW - Egtazic Acid

KW - Exosomes

KW - Humans

KW - Magnetic Fields

KW - Organelles

KW - Porosity

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.bbrc.2012.12.012

DO - 10.1016/j.bbrc.2012.12.012

M3 - Article

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SN - 0006-291X

VL - 430

SP - 470

EP - 475

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells

Abstract

Keywords

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