Putative DNA quadruplex formation within the human c-kit oncogene

Sarah Rankin, Anthony P. Reszka, Julian Huppert, Mire Zloh, Gary N. Parkinson, Alan K. Todd, Sylvain Ladame, Shankar Balasubramanian, Stephen Neidle

Research output: Contribution to journalArticlepeer-review

449 Citations (Scopus)


The DNA sequence, d(AGGGAGGGCGCTGGGAGGAGGG), occurs within the promoter region of the c-kit oncogene. We show here, using a combination of NMR, circular dichroism, and melting temperature measurements, that this sequence forms a four-stranded quadruplex structure under physiological conditions. Variations in the sequences that intervene between the guanine tracts have been examined, and surprisingly, none of these modified sequences forms a quadruplex arrangement under these conditions. This suggests that the occurrence of quadruplex-forming sequences within the human and other genomes is less than was hitherto expected. The c-kit quadruplex may be a new target for therapeutic intervention in cancers where there is elevated expression of the c-kit gene.
Original languageEnglish
Pages (from-to)10584-9
Number of pages6
JournalJournal of the American Chemical Society
Issue number30
Publication statusPublished - 2005


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