Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration

Andrei I Tarasov

Research output: Contribution to journalArticlepeer-review

Abstract

The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.

Original languageEnglish
Pages (from-to)151-64
Number of pages14
JournalMethods in Molecular Biology
Volume491
DOIs
Publication statusPublished - 2008

Keywords

  • Animals
  • Bacterial Proteins/pharmacology
  • Bacterial Toxins/pharmacology
  • Cell Adhesion/drug effects
  • Cell Membrane/physiology
  • Cell Membrane Permeability/drug effects
  • Cells, Cultured
  • Electrophysiology/methods
  • Hemolysin Proteins/pharmacology
  • Insulin-Secreting Cells/cytology
  • KATP Channels/drug effects
  • Mice
  • Patch-Clamp Techniques
  • Streptolysins/pharmacology

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