Abstract
The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.
Original language | English |
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Pages (from-to) | 151-64 |
Number of pages | 14 |
Journal | Methods in Molecular Biology |
Volume | 491 |
DOIs | |
Publication status | Published - 2008 |
Keywords
- Animals
- Bacterial Proteins/pharmacology
- Bacterial Toxins/pharmacology
- Cell Adhesion/drug effects
- Cell Membrane/physiology
- Cell Membrane Permeability/drug effects
- Cells, Cultured
- Electrophysiology/methods
- Hemolysin Proteins/pharmacology
- Insulin-Secreting Cells/cytology
- KATP Channels/drug effects
- Mice
- Patch-Clamp Techniques
- Streptolysins/pharmacology