Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration

Andrei I Tarasov

doi:10.1007/978-1-59745-526-8_12

Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration

Andrei I Tarasov

Research output: Contribution to journal › Article › peer-review

Abstract

The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.

Original language	English
Pages (from-to)	151-64
Number of pages	14
Journal	Methods in Molecular Biology
Volume	491
DOIs	https://doi.org/10.1007/978-1-59745-526-8_12
Publication status	Published - 2008

Keywords

Animals
Bacterial Proteins/pharmacology
Bacterial Toxins/pharmacology
Cell Adhesion/drug effects
Cell Membrane/physiology
Cell Membrane Permeability/drug effects
Cells, Cultured
Electrophysiology/methods
Hemolysin Proteins/pharmacology
Insulin-Secreting Cells/cytology
KATP Channels/drug effects
Mice
Patch-Clamp Techniques
Streptolysins/pharmacology

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title = "Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration",

abstract = "The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.",

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author = "Tarasov, \{Andrei I\}",

note = "{\textcopyright} Humana Press, a part of Springer Science+Business Media, LLC 2008",

year = "2008",

doi = "10.1007/978-1-59745-526-8\_12",

language = "English",

volume = "491",

pages = "151--64",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

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T1 - Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration

AU - Tarasov, Andrei I

N1 - © Humana Press, a part of Springer Science+Business Media, LLC 2008

PY - 2008

Y1 - 2008

N2 - The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.

AB - The functional activity of ion channels is affected by their local environment, and for certain types of channels this local effect is critical. This chapter describes a method of recording the activity of ATP-sensitive K(+) channels, expressed on the plasma membrane of pancreatic beta-cells, without destroying the beta-cell architecture. In the open-cell cell-attached patch-clamp configuration, ligands and effectors of these metabolism-dependent channels are delivered to their cytoplasmic side via pores in the plasma membrane produced by bacterial toxins.

KW - Animals

KW - Bacterial Proteins/pharmacology

KW - Bacterial Toxins/pharmacology

KW - Cell Adhesion/drug effects

KW - Cell Membrane/physiology

KW - Cell Membrane Permeability/drug effects

KW - Cells, Cultured

KW - Electrophysiology/methods

KW - Hemolysin Proteins/pharmacology

KW - Insulin-Secreting Cells/cytology

KW - KATP Channels/drug effects

KW - Mice

KW - Patch-Clamp Techniques

KW - Streptolysins/pharmacology

U2 - 10.1007/978-1-59745-526-8_12

DO - 10.1007/978-1-59745-526-8_12

M3 - Article

C2 - 18998091

SN - 1064-3745

VL - 491

SP - 151

EP - 164

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Recording the activity of ATP-sensitive K(+) channels in open-cell cell-attached configuration

Abstract

Keywords

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