Revealing the density of encoded functions in a viral RNA

N. Patel, E.C. Dykeman, Robert H.A. Coutts, G.P. Lomonossoff, D.J. Rowlands, S.E.V. Phillips, N. Ranson, R. Twarock, R. Tuma, P.G. Stockley

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)
63 Downloads (Pure)

Abstract

We present direct experimental evidence that assembly of a single-stranded RNA virus occurs via a packaging signal-mediated mechanism. We show that the sequences of coat protein recognition motifs within multiple, dispersed, putative RNA packaging signals, as well as their relative spacing within a genomic fragment, act collectively to influence the fidelity and yield of capsid self-assembly in vitro. These experiments confirm that the selective advantages for viral yield and encapsidation specificity, predicted from previous modeling of packaging signal-mediated assembly, are found in Nature. Regions of the genome that act as packaging signals also function in translational and transcriptional enhancement, as well as directly coding for the coat protein, highlighting the density of encoded functions within the viral RNA. Assembly and gene expression are therefore direct molecular competitors for different functional folds of the same RNA sequence. The strongest packaging signal in the test fragment, encodes a region of the coat protein that undergoes a conformational change upon contact with packaging signals. A similar phenomenon occurs in other RNA viruses for which packaging signals are known. These contacts hint at an even deeper density of encoded functions in viral RNA, which if confirmed, would have profound consequences for the evolution of this class of pathogens
Original languageEnglish
Pages (from-to)2227-2232
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number7
DOIs
Publication statusPublished - 17 Feb 2015

Keywords

  • virus assembly
  • single-molecule fluorescence correlation spectroscopy
  • satellite tobacco necrosis virus
  • packaging signal

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