RNA polymerase activity during breakage of seed dormancy by low temperature treatment of fruits of Acer platanoides (Norway maple)

R.J. Slater, J.A. Bryant

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Endogenous RNA polymerase activity has been characterized in nuclei isolated from embryo axes of Acer platanoides. Optimal activity was recorded at 4·0 mol m MgCl and 50 mol m (NH) SO and total activity could be inhibited by up to 30% by α-amanitin. Stratification of fruits leads to a stimulation of RNA polymerase activity. A minimum of 3 d cold treatment is required with at least 3-fold stimulation recorded after 10 d at 4°C. The increased enzyme activity is resistant to α-amanitin suggesting an effect on RNA polymerase I.
Original languageEnglish
Pages (from-to)1026-1032
Number of pages7
JournalJournal of Experimental Botany
Volume38
Issue number6
DOIs
Publication statusPublished - 1 Jun 1987

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