Abstract
In this work we report for the first time on a repertoire of valving technologies which are combined to enable automated purification of total RNA from cell homogenate. Process control is implemented using rotational-pulse actuated dissolvable-film (DF) valves; where the order of valve actuation is determined by the disc architecture while the timing of valve actuation is governed by pulses in the spin rate. Selective liquid routing is enabled by combining a heavy, inert and immiscible liquid plug with a DF. The combination of these technologies enables bead-based extraction of amplifiable RNA, with a yield comparable to gold-standard bench-top protocols.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1437-1439 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Keywords
- Dissolvable films
- Lab-on-a-Disc
- Nucleic acid
- Sample preparation
- Valving