Silica passivated conjugated polymer nanoparticles for biological imaging applications

Struan Bourke; Laura Urbano; Antoni Olona; Ferran Valderrama; Lea Ann Dailey; Mark A. Green

doi:10.1117/12.2252035

Silica passivated conjugated polymer nanoparticles for biological imaging applications

Struan Bourke, Laura Urbano, Antoni Olona, Ferran Valderrama, Lea Ann Dailey, Mark A. Green

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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Abstract

Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

Original language	English
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX
Publisher	SPIE (International Society for Optics and Phonotics)
Volume	10079
DOIs	https://doi.org/10.1117/12.2252035
Publication status	Published - 21 Feb 2017

Keywords

Biological imaging, Cancer imaging, Conjugated polymers, Nanoparticles, Silica shells

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10.1117/12.2252035

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Bourke, S., Urbano, L., Olona, A., Valderrama, F., Ann Dailey, L., & Green, M. A. (2017). Silica passivated conjugated polymer nanoparticles for biological imaging applications. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX (Vol. 10079). SPIE (International Society for Optics and Phonotics). https://doi.org/10.1117/12.2252035

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abstract = "Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.",

keywords = "Biological imaging, Cancer imaging, Conjugated polymers, Nanoparticles, Silica shells",

author = "Struan Bourke and Laura Urbano and Antoni Olona and Ferran Valderrama and {Ann Dailey}, Lea and Green, {Mark A.}",

note = "{\textcopyright} 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).",

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language = "English",

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Silica passivated conjugated polymer nanoparticles for biological imaging applications. / Bourke, Struan; Urbano, Laura; Olona, Antoni et al.
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. Vol. 10079 SPIE (International Society for Optics and Phonotics), 2017.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Silica passivated conjugated polymer nanoparticles for biological imaging applications

AU - Bourke, Struan

AU - Urbano, Laura

AU - Olona, Antoni

AU - Valderrama, Ferran

AU - Ann Dailey, Lea

AU - Green, Mark A.

PY - 2017/2/21

Y1 - 2017/2/21

N2 - Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

AB - Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

KW - Biological imaging, Cancer imaging, Conjugated polymers, Nanoparticles, Silica shells

U2 - 10.1117/12.2252035

DO - 10.1117/12.2252035

M3 - Chapter

VL - 10079

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX

PB - SPIE (International Society for Optics and Phonotics)

ER -

Silica passivated conjugated polymer nanoparticles for biological imaging applications

Abstract

Keywords

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