Solute and matric potential stress on Penicillium verrucosum: Impact on growth, gene expression and ochratoxin A p production

Shaimaa Abdelmohsen, Carol Verheecke-Vaessen, Esther Garcia-Cela, Angel Medina, Naresh Magan

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
8 Downloads (Pure)

Abstract

Penicillium verrucosum survives in soil and on cereal debris. It colonises grain during harvesting, drying and storage. There is no information on the relative tolerance of P. verrucosum to solute and matric stress in terms of colonisation, or on the biosynthetic toxin gene clusters or ochratoxin A (OTA) production. The objectives were to examine the effect of ionic and non-ionic solute and matric potential stress on (a) growth, (b) expression of two toxin biosynthetic genes otapksPV and otanrpsPV, and (c) OTA production by a strain of P. verrucosum. Optimum growth and OTA production were at-7.0 MPa (= 0.95 water activity, aw) and-1.4 MPa (= 0.99 aw), respectively, regardless of whether solute (Ψs) or matric (Ψm) stress was imposed. P. verrucosum was more sensitive to ionic solute stress (NaCl) with no growth at-19.6 MPa (=0.86 aw) while growth still occurred in the non-ionic solute (glycerol) and matric stress treatments. Relative gene expression of the biosynthetic genes using PCR (RT-qPCR) showed that the otapksPV gene was expressed over a wide range of ionic/non-ionic solute stress conditions (-1.4 to-14.0 MPa; = 0.99-0.90 aw). The highest expression was in the non-ionic Ψs stress treatments at-7.0 MPa (= 0.95 aw). However, the otanrpsPV gene was significantly up regulated under Ψm stress, especially with freely available water (-1.4 MPa = 0.99 aw). OTA production was significantly decreased as Ψs or Ψm stress were imposed. Limited OTA production occurred in the driest treatments under Ψs and Ψm stress respectively. The impact of these two types of stresses on the growth of P. verrucosum was quite different from that for OTA production. The results are discussed in the context of the life cycle and ecological characteristics of this species in contaminating cereals with OTA in the post-harvest phase of the cereal chain.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalWorld Mycotoxin Journal
Volume13
Issue number3
Early online date27 Mar 2020
DOIs
Publication statusE-pub ahead of print - 27 Mar 2020

Keywords

  • Biosynthetic genes
  • Matric potential
  • Mycotoxin
  • Ochratoxin a
  • Penicillium verrucosum
  • Qpcr
  • Solute potential
  • Water activity

Fingerprint

Dive into the research topics of 'Solute and matric potential stress on Penicillium verrucosum: Impact on growth, gene expression and ochratoxin A p production'. Together they form a unique fingerprint.

Cite this