Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells

Kerenza L. Njoh; Laurence H. Patterson; Mire Zloh; Marie Wiltshire; Janet Fisher; Sally Chappell; Simon Ameer-Beg; Yanhong Bai; Daniel Matthews; Rachel J. Errington; Paul J. Smith

doi:10.1002/cyto.a.20308

Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells

Kerenza L. Njoh, Laurence H. Patterson, Mire Zloh, Marie Wiltshire, Janet Fisher, Sally Chappell, Simon Ameer-Beg, Yanhong Bai, Daniel Matthews, Rachel J. Errington, Paul J. Smith

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.

Original language	English
Pages (from-to)	805-14
Number of pages	10
Journal	Cytometry Part A
Volume	69
Issue number	8
DOIs	https://doi.org/10.1002/cyto.a.20308
Publication status	Published - Aug 2006

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title = "Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells",

abstract = "We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.",

author = "Njoh, {Kerenza L.} and Patterson, {Laurence H.} and Mire Zloh and Marie Wiltshire and Janet Fisher and Sally Chappell and Simon Ameer-Beg and Yanhong Bai and Daniel Matthews and Errington, {Rachel J.} and Smith, {Paul J.}",

year = "2006",

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doi = "10.1002/cyto.a.20308",

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AU - Njoh, Kerenza L.

AU - Patterson, Laurence H.

AU - Zloh, Mire

AU - Wiltshire, Marie

AU - Fisher, Janet

AU - Chappell, Sally

AU - Ameer-Beg, Simon

AU - Bai, Yanhong

AU - Matthews, Daniel

AU - Errington, Rachel J.

AU - Smith, Paul J.

PY - 2006/8

Y1 - 2006/8

N2 - We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.

AB - We report on the potential DNA binding modes and spectral characteristics of the cell-permeant far red fluorescent DNA dye, DRAQ5, in solution and bound within intact cells. Our aim was to determine the constraints for its use in flow cytometry and bioimaging.

U2 - 10.1002/cyto.a.20308

DO - 10.1002/cyto.a.20308

M3 - Article

C2 - 16969814

SN - 1552-4922

VL - 69

SP - 805

EP - 814

JO - Cytometry Part A

JF - Cytometry Part A

IS - 8

ER -

Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells

Abstract

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