Abstract
The spread of BNYVV in P. betaefBNYVV -infected sugarbeet was directly monitored by ELISA and tissue print immuno blot detection, whilst that of P. betae was indirectly monitored using the zoospore antagonist Bacillus subtilis. These studies revealed· a fungus-related component of rhizomania
resistance in one Gennan sugarbeet variety. Nucleic acid based P. betae detection methods have also been developed for direct detection of fungus development. It was shown that quantitative development and distribution of P .. betae in roots could be followed by dot blot hybridization and tissue print
hybridization blotting respectively. BNYVV detection methods can now be complemented by P. betae detection methods, making it possible to differentiate between virus- and fungus-related rhizomania resistance mechanisms in sugarbeet varieties
resistance in one Gennan sugarbeet variety. Nucleic acid based P. betae detection methods have also been developed for direct detection of fungus development. It was shown that quantitative development and distribution of P .. betae in roots could be followed by dot blot hybridization and tissue print
hybridization blotting respectively. BNYVV detection methods can now be complemented by P. betae detection methods, making it possible to differentiate between virus- and fungus-related rhizomania resistance mechanisms in sugarbeet varieties
Original language | English |
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Title of host publication | Procs 3rd Symp. of the Int. Working Group on Plant Viruses with Fungal Vectors |
Editors | J.L. Sherwood, C.M. Rush |
Publisher | American Society of Sugar Beet Technologists |
Pages | 177-180 |
ISBN (Print) | 0-926195-02-6 |
Publication status | Published - 1996 |
Event | 3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors - Dundee, United Kingdom Duration: 6 Aug 1996 → 7 Aug 1996 |
Conference
Conference | 3rd Symposium of Int Working Group on Plant Viruses with Fungal Vectors |
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Country/Territory | United Kingdom |
City | Dundee |
Period | 6/08/96 → 7/08/96 |