TY - JOUR
T1 - Studies on the Detection, Expression, Glycosylation, Dimerization, and Ligand Binding Properties of Mouse Siglec-E
AU - Siddiqui, Shoib
AU - Schwarz, Flavio
AU - Springer, Stevan
AU - Khedri, Zahra
AU - Yu, Hai
AU - Deng, Lingquan
AU - Verhagen, Andrea
AU - Naito-Matsui, Yuko
AU - Jiang, Weiping
AU - Kim, Daniel
AU - Zhou, Jie
AU - Ding, Beibei
AU - Chen, Xi
AU - Varki, Nissi
AU - Varki, Ajit
N1 - © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A. This is an Open Access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/)
PY - 2017/1/20
Y1 - 2017/1/20
N2 - CD33-related Siglecs are a family of proteins widely expressed on innate immune cells. Binding of sialylated glycans or other ligands triggers signals that inhibit or activate inflammation. Immunomodulation by Siglecs has been extensively studied, but relationships between structure and functions are poorly explored. Here we present new data relating to the structure and function of Siglec-E, the major CD33-related Siglec expressed on mouse neutrophils, monocytes, macrophages, and dendritic cells. We generated nine new rat monoclonal antibodies specific to mouse Siglec-E, with no cross-reactivity to Siglec-F. Although all antibodies detected Siglec-E on transfected human HEK-293T cells, only two reacted with mouse bone marrow neutrophils by flow cytometry and on spleen sections by immunohistochemistry. Moreover, whereas all antibodies recognized Siglec-E-Fc on immunoblots, binding was dependent on intact disulfide bonds and N-glycans, and only two antibodies recognized native Siglec-E within spleen lysates. Thus, we further investigated the impact of Siglec-E homodimerization. Homology-based structural modeling predicted a cysteine residue (Cys-298) in position to form a disulfide bridge between two Siglec-E polypeptides. Mutagenesis of Cys-298 confirmed its role in dimerization. In keeping with the high level of 9-O-acetylation found in mice, sialoglycan array studies indicate that this modification has complex effects on recognition by Siglec-E, in relationship to the underlying structures. However, we found no differences in phosphorylation or SHP-1 recruitment between dimeric and monomeric Siglec-E expressed on HEK293A cells. Phylogenomic analyses predicted that only some human and mouse Siglecs form disulfide-linked dimers. Notably, Siglec-9, the functionally equivalent human paralog of Siglec-E, occurs as a monomer.
AB - CD33-related Siglecs are a family of proteins widely expressed on innate immune cells. Binding of sialylated glycans or other ligands triggers signals that inhibit or activate inflammation. Immunomodulation by Siglecs has been extensively studied, but relationships between structure and functions are poorly explored. Here we present new data relating to the structure and function of Siglec-E, the major CD33-related Siglec expressed on mouse neutrophils, monocytes, macrophages, and dendritic cells. We generated nine new rat monoclonal antibodies specific to mouse Siglec-E, with no cross-reactivity to Siglec-F. Although all antibodies detected Siglec-E on transfected human HEK-293T cells, only two reacted with mouse bone marrow neutrophils by flow cytometry and on spleen sections by immunohistochemistry. Moreover, whereas all antibodies recognized Siglec-E-Fc on immunoblots, binding was dependent on intact disulfide bonds and N-glycans, and only two antibodies recognized native Siglec-E within spleen lysates. Thus, we further investigated the impact of Siglec-E homodimerization. Homology-based structural modeling predicted a cysteine residue (Cys-298) in position to form a disulfide bridge between two Siglec-E polypeptides. Mutagenesis of Cys-298 confirmed its role in dimerization. In keeping with the high level of 9-O-acetylation found in mice, sialoglycan array studies indicate that this modification has complex effects on recognition by Siglec-E, in relationship to the underlying structures. However, we found no differences in phosphorylation or SHP-1 recruitment between dimeric and monomeric Siglec-E expressed on HEK293A cells. Phylogenomic analyses predicted that only some human and mouse Siglecs form disulfide-linked dimers. Notably, Siglec-9, the functionally equivalent human paralog of Siglec-E, occurs as a monomer.
KW - Amino Acid Substitution
KW - Animals
KW - Antibodies/chemistry
KW - Antigens, CD/chemistry
KW - Antigens, Differentiation, B-Lymphocyte/chemistry
KW - Dendritic Cells/cytology
KW - Gene Expression Regulation/physiology
KW - Glycosylation
KW - Humans
KW - Macrophages/cytology
KW - Mice
KW - Mice, Knockout
KW - Monocytes/cytology
KW - Mutagenesis
KW - Mutation, Missense
KW - Neutrophils/cytology
KW - Protein Multimerization/physiology
KW - Protein Tyrosine Phosphatase, Non-Receptor Type 6
KW - Rats
KW - Rats, Inbred Lew
KW - Sialic Acid Binding Immunoglobulin-like Lectins/chemistry
U2 - 10.1074/jbc.M116.738351
DO - 10.1074/jbc.M116.738351
M3 - Article
C2 - 27920204
SN - 0021-9258
VL - 292
SP - 1029
EP - 1037
JO - The Journal of biological chemistry
JF - The Journal of biological chemistry
IS - 3
ER -