Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

Gabriel C Uguru, Madhav Mondhe, Shan Goh, Andrew Hesketh, Mervyn J Bibb, Liam Good, James E M Stach

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)
14 Downloads (Pure)

Abstract

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.

Original languageEnglish
Pages (from-to)e67509
JournalPLoS ONE
Volume8
Issue number6
DOIs
Publication statusPublished - 27 Jun 2013

Keywords

  • Journal Article

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