Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

Gabriel C Uguru; Madhav Mondhe; Shan Goh; Andrew Hesketh; Mervyn J Bibb; Liam Good; James E M Stach

doi:10.1371/journal.pone.0067509

Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

Gabriel C Uguru, Madhav Mondhe, Shan Goh, Andrew Hesketh, Mervyn J Bibb, Liam Good, James E M Stach

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Abstract

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.

Original language	English
Pages (from-to)	e67509
Journal	PLoS ONE
Volume	8
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0067509
Publication status	Published - 27 Jun 2013

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journal.pone.0067509Final published version, 3.4 MBLicence: CC BY

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title = "Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)",

abstract = "We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.",

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AU - Uguru, Gabriel C

AU - Mondhe, Madhav

AU - Goh, Shan

AU - Hesketh, Andrew

AU - Bibb, Mervyn J

AU - Good, Liam

AU - Stach, James E M

PY - 2013/6/27

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AB - We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.

KW - Journal Article

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Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

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