The development of species-specific antisense peptide nucleic acid method for the treatment and detection of viable Salmonella

Oluwawemimo Adebowale, Shan Goh, Liam Good

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
27 Downloads (Pure)

Abstract

Genotypic based detection methods using specific target sites in the pathogen genome can complement phenotypic identification. We report the development of species-specific antisense peptide nucleic acid (PNA) combined with selective and differential enrichment growth conditions for Salmonella treatment and detection. An antisense PNA oligomer targeting the Salmonella ftsZ gene and conjugated with a cell-penetrating peptide ((KFF)3K) was exploited to probe bacteria cultured in three different growth media (Muller Hinton broth (MHB), Rappaport-Vassiliadis Soya Peptone Broth (RVS, Oxoid), and in-house modified Rappaport-Vassiliadis Soya Peptone Broths (mRVSs). Also, water and milk artificially contaminated with bacteria were probed. Antisense PNA provided detectable changes in Salmonella growth and morphology in all media and artificially contaminated matrices except RVS. Salmonella was detected as elongated cells. On the contrary, treated Escherichia coli did not elongate, providing evidence of differentiation and selectivity for Salmonella. Similarly, Salmonella probed with mismatched PNAs did not elongate. Antisense oligomers targeted ftsZ mRNA in combination with selective growth conditions can provide a detection strategy for viable Salmonella in a single reaction, and act as a potential tool for bacteria detection in real food and environmental samples.
Original languageEnglish
Article numbere04110
Pages (from-to)e04110
Number of pages9
JournalHeliyon
Volume6
Issue number6
DOIs
Publication statusPublished - Jun 2020

Keywords

  • Antisense
  • Health sciences
  • Infectious disease
  • Microbial diagnostics
  • Microbiology
  • Peptide nucleic acids
  • Public health
  • Salmonella
  • Veterinary medicine
  • ftsZ

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