Abstract
Host microvesicles (MVs) help pathogens, such as the intracellular
parasite, Trypanosoma cruzi, to evade complement attack. We have
now found that the infectious metacyclic trypomastigote forms, by
interacting with host integrins, lipid rafts and stretch activated
channels, stimulate a calcium-mediated depolymerization of the
actin cytoskeleton and MV release. The release of MVs in turn
stimulates a lysosomal repair mechanism in the host cell, to plug the
breach in the plasma membrane. By using specific inhibitors of
lysosomal exocytosis and both pharmacological and siRNA-mediated
inhibition of microvesicle release, we describe a novel entry
mechanism by which the parasite opportunistically takes advantage
of a host membrane repair mechanism, to execute entry before
membrane integrity is fully restored.
Funded by: The Royal Society (London) to JI Brazilian Ministry of
Education to IC
parasite, Trypanosoma cruzi, to evade complement attack. We have
now found that the infectious metacyclic trypomastigote forms, by
interacting with host integrins, lipid rafts and stretch activated
channels, stimulate a calcium-mediated depolymerization of the
actin cytoskeleton and MV release. The release of MVs in turn
stimulates a lysosomal repair mechanism in the host cell, to plug the
breach in the plasma membrane. By using specific inhibitors of
lysosomal exocytosis and both pharmacological and siRNA-mediated
inhibition of microvesicle release, we describe a novel entry
mechanism by which the parasite opportunistically takes advantage
of a host membrane repair mechanism, to execute entry before
membrane integrity is fully restored.
Funded by: The Royal Society (London) to JI Brazilian Ministry of
Education to IC
| Original language | English |
|---|---|
| Pages | 21 |
| Number of pages | 22 |
| Publication status | Published - 15 Apr 2015 |
| Event | International Society for Extracellular Vesicles 1st annual meeting: ISEV meeting, 2012, Gothenburg - University of Gothenburg, Gothenburg, Sweden Duration: 18 Apr 2012 → 21 Apr 2012 https://www.gu.se/digitalAssets/1367/1367704_scientific-program-gothenburg-april-2012-finalmwii--1-.pdf |
Conference
| Conference | International Society for Extracellular Vesicles 1st annual meeting |
|---|---|
| Abbreviated title | ISEV, 2012 |
| Country/Territory | Sweden |
| City | Gothenburg |
| Period | 18/04/12 → 21/04/12 |
| Internet address |