The non-genomic effects of the PPARβγ agonist GW0742 on streptozotocin treated rat aorta

Noelia Perez-Diaz, Igor Pushkarsky, Nadia Oweis, Lisa Lione, Louise S Mackenzie

Research output: Contribution to journalArticlepeer-review

64 Downloads (Pure)


Background: The ubiquitous nuclear receptor PPARβ/δ is increasingly being studied in regards to numerous diseases including diabetes following on the finding that PPARβ/δ agonist GW0742 controls Type 1 Diabetes in rats. Studies have shown that GW0742 has off target, non- PPARβ/δ effects in the cell although there are some key questions that remain to be addressed in respect to the significance of this control on vascular tone. Methods: Using isometric organ baths, rat aorta rings were exposed to ROCK inhibitors and the changes in contraction and dilation measured. Results: Our data shows that the PPARβ/δ agonist GW0742 (10 -7M) inhibits contractile responses to U46619 and phenylephrine, and that these responses are similar in normal and Streptozotocin (STZ) diabetic rat aorta. ROCK inhibitors Fasudil and Y27632 significantly reduced GW0742 mediated dilation of naïve rat aorta, but Fasudil had no effect on GW0742 dilation in STZ diabetic rat aorta. In contrast, STZ diabetic rat aorta pre-contracted with high [K +] Krebs lacked a dilatory response to GW0742, which taken together indicates that the mechanism of action of GW0742 mediated dilation changes in the diabetic state compared to non-diabetic state. Conclusion: This is the first direct evidence demonstrating the non- PPARβ/δ effect of GW0742 on contraction is irrespective to the diabetic state, and that GW0742 has the potential to induce vasodilation via multiple off-target mechanisms.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalCurrent Molecular Pharmacology
Issue number2
Early online date31 Dec 2016
Publication statusPublished - 1 May 2018


  • Artery
  • Diabetes
  • Non-Genomic Effects
  • PPAR
  • Rat Arohta
  • ROCK
  • ST2
  • Vasodilation


Dive into the research topics of 'The non-genomic effects of the PPARβγ agonist GW0742 on streptozotocin treated rat aorta'. Together they form a unique fingerprint.

Cite this