The osmotic characteristics of human fetal liver-derived hematopoietic stem cell candidates

A I Tarasov, A Yu Petrenko, D R E Jones

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Hematopoietic stem cells derived from fetal liver have promising therapeutic potential for allotransplantation but require a specific protocol to minimize the damage produced by cryopreservation procedures. In this study, a fundamental approach was applied for designing a cell preservation protocol. To this end, the biophysical characteristics that describe the osmotic reaction of CD34(+)CD38(-) human fetal liver stem cell candidates were studied using fluorescent microscopy. The osmotically inactive volume of the stem cell candidates was determined as 48% of the isotonic volume. The permeability coefficients for water and Me(2)SO were determined at T = +22 degree C: L(p) = 0.27 +/- 0.03 microm x min(-1)atm(-1), P(Me(2)SO)) = 2.09 +/- 0.85 x 10 (-4) cm x min(-1), sigma (Me(2)SO)) = 0.63 +/- 0.03 and at T = +12 degree C: L(p) = 0.15 +/-0.02 microm x min(-1)atm(-1), P(Me(2)SO)) = 6.44 +/-1.42 x 10 (-5) cm x min(-1), sigma (Me(2)SO)) = 0.46 +/- 0.05. The results obtained suggest that post-hypertonic and hypotonic stress are the possible reasons for damage to a CD34(+)CD38(-) cell during the cryopreservation procedure.

Original languageEnglish
Pages (from-to)333-340
Number of pages8
Issue number3
Early online date6 May 2004
Publication statusPublished - 1 Jun 2004


  • AC133 Antigen
  • Antigens, CD
  • Antigens, CD34/analysis
  • Blood Preservation
  • Cell Membrane Permeability/drug effects
  • Cell Size
  • Cryopreservation/methods
  • Cryoprotective Agents/pharmacology
  • Dimethyl Sulfoxide/pharmacology
  • Glycoproteins/analysis
  • Hematopoietic Stem Cells
  • Humans
  • Immunophenotyping
  • Leukocyte Common Antigens/analysis
  • Liver/cytology
  • Osmosis
  • Peptides/analysis
  • Temperature
  • Water/metabolism


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