The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles

David Y.S. Chau, Naing L. Tint, Russell J. Collighan, Martin Griffin, Harminder S. Dua, Kevin M. Shakesheff, Felicity R. A. J. Rose

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
72 Downloads (Pure)

Abstract

Aims To demonstrate the potential use of in vitro poly (lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy.
Methods PLGA microparticles (diameter 10-60 mu m) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous.
Results Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium.
Conclusion The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.

Original languageEnglish
Pages (from-to)648-653
Number of pages6
JournalBritish Journal of Ophthalmology
Volume94
Issue number5
DOIs
Publication statusPublished - May 2010

Keywords

  • PREFERRED SUBSTRATE SEQUENCES
  • DISPLAYED PEPTIDE LIBRARY
  • TRIAMCINOLONE ACETONIDE
  • ANTERIOR-CHAMBER
  • MICROBIAL TRANSGLUTAMINASE
  • TISSUE TRANSGLUTAMINASE
  • CATARACT-SURGERY
  • MICROSPHERES
  • CAPSULE
  • CELLS

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