University of Hertfordshire

By the same authors

Standard

. Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma. / Ansa-Addo, Ephraim A.; Stratton, Dan; Jorfi, Samireh; Kholia, Sharad; Krige, Lizelle; Lange, Sigrun; Inal, Jameel.

2012. 14-14 Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.

Research output: Contribution to conferenceAbstractpeer-review

Harvard

Ansa-Addo, EA, Stratton, D, Jorfi, S, Kholia, S, Krige, L, Lange, S & Inal, J 2012, '. Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma', International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden, 18/04/12 - 21/04/12 pp. 14-14.

APA

Ansa-Addo, E. A., Stratton, D., Jorfi, S., Kholia, S., Krige, L., Lange, S., & Inal, J. (2012). . Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma. 14-14. Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.

Vancouver

Ansa-Addo EA, Stratton D, Jorfi S, Kholia S, Krige L, Lange S et al. . Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma. 2012. Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.

Author

Ansa-Addo, Ephraim A. ; Stratton, Dan ; Jorfi, Samireh ; Kholia, Sharad ; Krige, Lizelle ; Lange, Sigrun ; Inal, Jameel. / . Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma. Abstract from International Society for Extracellular Vesicles 1st annual meeting, Gothenburg, Sweden.1 p.

Bibtex

@conference{e5e144613c63454abb683650a8cd8eda,
title = ". Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma",
abstract = "Current protocols for the isolation of microvesicles (MVs) andexosomes, which in the main focus on differential centrifugation,vary considerably. In an attempt to set a new standard, we describea filtration protocol for isolating phosphatidylserine-positive MVs(larger than 200 nm in diameter) and exosomes. The keypreparative step to successfully isolate both MVs and exosomesto a high degree of purity was a gentle sonication to break upexosome clumps. Filtration through a 200 nm pore size Milliporefilter allowed for collection of exosomes in the filtrate. The largerMVs could then be recovered from the filter. Annexin V-PE MVswere sized and quantified using Polysciences Polybead Microspheres(200 nm) and BDTrucount tubes, respectively, on a FACSCaliburTM flow cytometer. The normal reference range from normalhuman donors was found to be 0.512.82105 MVs/ml. Freeze/thawing of samples had little effect on MV counts and with age MVlevels seemed only marginally reduced. Fasting status also affectedMV levels, appearing up to 3-fold higher in fasting individuals.Smokers had reduced MV counts and nicotine reduced MV releasefrom THP-1 cells.Funded by: Cellular and Molecular Immunology Research Centre/Faculty of Life Sciences and Hammersmith Medicines Research. S.K.received a VC scholarship (London metropolitan University).",
author = "Ansa-Addo, {Ephraim A.} and Dan Stratton and Samireh Jorfi and Sharad Kholia and Lizelle Krige and Sigrun Lange and Jameel Inal",
year = "2012",
month = apr,
day = "15",
language = "English",
pages = "14--14",
note = "International Society for Extracellular Vesicles 1st annual meeting : ISEV meeting, 2012, Gothenburg, ISEV, 2012 ; Conference date: 18-04-2012 Through 21-04-2012",
url = "https://www.gu.se/digitalAssets/1367/1367704_scientific-program-gothenburg-april-2012-finalmwii--1-.pdf",

}

RIS

TY - CONF

T1 - . Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma

AU - Ansa-Addo, Ephraim A.

AU - Stratton, Dan

AU - Jorfi, Samireh

AU - Kholia, Sharad

AU - Krige, Lizelle

AU - Lange, Sigrun

AU - Inal, Jameel

PY - 2012/4/15

Y1 - 2012/4/15

N2 - Current protocols for the isolation of microvesicles (MVs) andexosomes, which in the main focus on differential centrifugation,vary considerably. In an attempt to set a new standard, we describea filtration protocol for isolating phosphatidylserine-positive MVs(larger than 200 nm in diameter) and exosomes. The keypreparative step to successfully isolate both MVs and exosomesto a high degree of purity was a gentle sonication to break upexosome clumps. Filtration through a 200 nm pore size Milliporefilter allowed for collection of exosomes in the filtrate. The largerMVs could then be recovered from the filter. Annexin V-PE MVswere sized and quantified using Polysciences Polybead Microspheres(200 nm) and BDTrucount tubes, respectively, on a FACSCaliburTM flow cytometer. The normal reference range from normalhuman donors was found to be 0.512.82105 MVs/ml. Freeze/thawing of samples had little effect on MV counts and with age MVlevels seemed only marginally reduced. Fasting status also affectedMV levels, appearing up to 3-fold higher in fasting individuals.Smokers had reduced MV counts and nicotine reduced MV releasefrom THP-1 cells.Funded by: Cellular and Molecular Immunology Research Centre/Faculty of Life Sciences and Hammersmith Medicines Research. S.K.received a VC scholarship (London metropolitan University).

AB - Current protocols for the isolation of microvesicles (MVs) andexosomes, which in the main focus on differential centrifugation,vary considerably. In an attempt to set a new standard, we describea filtration protocol for isolating phosphatidylserine-positive MVs(larger than 200 nm in diameter) and exosomes. The keypreparative step to successfully isolate both MVs and exosomesto a high degree of purity was a gentle sonication to break upexosome clumps. Filtration through a 200 nm pore size Milliporefilter allowed for collection of exosomes in the filtrate. The largerMVs could then be recovered from the filter. Annexin V-PE MVswere sized and quantified using Polysciences Polybead Microspheres(200 nm) and BDTrucount tubes, respectively, on a FACSCaliburTM flow cytometer. The normal reference range from normalhuman donors was found to be 0.512.82105 MVs/ml. Freeze/thawing of samples had little effect on MV counts and with age MVlevels seemed only marginally reduced. Fasting status also affectedMV levels, appearing up to 3-fold higher in fasting individuals.Smokers had reduced MV counts and nicotine reduced MV releasefrom THP-1 cells.Funded by: Cellular and Molecular Immunology Research Centre/Faculty of Life Sciences and Hammersmith Medicines Research. S.K.received a VC scholarship (London metropolitan University).

M3 - Abstract

SP - 14

EP - 14

T2 - International Society for Extracellular Vesicles 1st annual meeting

Y2 - 18 April 2012 through 21 April 2012

ER -