University of Hertfordshire

Agarose gel electrophoresis of nucleic acids

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • D.R Williams
  • Ralph Rapley
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Original languageEnglish
Title of host publicationThe Nucleic Acids Protocols Handbook
EditorsR Rapley
Place of PublicationTotowa, NJ
PublisherHumana Press
Number of pages4
EditionPart II
ISBN (Electronic)978-1-59259-038-4
ISBN (Print)978-0-89603-459-4
Publication statusPublished - 2000


DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs


D. R. Williams and R. Rapley, 'Agarose gel electrophoresis of nucleic acids' in Ralph Rapley, ed., The Nucleic Acid Protocols Handbook (Totowa: Humana Press, 2000) ISBN: 978-0-89603-459-4, eISBN: 978-1-59259-038-4, DOI: 10.1385/1-59259-038-1:67 © 2000 Humana Press Inc., Totowa, NJ.

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