University of Hertfordshire

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An evaluation for the medium-term storage and viability of root cortex tissues stained with blue ink in the assessment of arbuscular mycorrhizal fungi. / Wilkes, Thomas; Warner, Douglas.

In: Access Microbiology, Vol. 3, No. 11, 000292, 30.11.2021.

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@article{c2c2fd78d7854b44960807c861e2a4fd,
title = "An evaluation for the medium-term storage and viability of root cortex tissues stained with blue ink in the assessment of arbuscular mycorrhizal fungi",
abstract = "Sheaffer blue ink is an effective method to stain arbuscular mycorrhizal (AM) fungi in a variety of plant species. It has, however, received criticism for its potential rapid degradation and short-term viability. The long and medium term storage and viability of stained samples has not, to date, been described for this particular staining method. This short communication reports on the viability of 730 samples stained with Sheaffer{\textregistered} blue ink stored for the duration of four years in microscope slide boxes out of direct sunlight. There was no significant difference in micrograph image quality and presence of stain between years as indicated by the number of AM fungal structures quantified. In conclusion Sheaffer{\textregistered} blue ink stain does not deteriorate in the medium term.",
keywords = "arbuscular mycorrhizal fungi, blue ink stain, root cortex, storage, degradation",
author = "Thomas Wilkes and Douglas Warner",
note = " {\textcopyright} 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. https://creativecommons.org/licenses/by/4.0/",
year = "2021",
month = nov,
day = "30",
doi = "10.1099/acmi.0.000292",
language = "English",
volume = "3",
journal = "Access Microbiology",
issn = "2516-8290",
publisher = "Microbiology Society",
number = "11",

}

RIS

TY - JOUR

T1 - An evaluation for the medium-term storage and viability of root cortex tissues stained with blue ink in the assessment of arbuscular mycorrhizal fungi

AU - Wilkes, Thomas

AU - Warner, Douglas

N1 - © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. https://creativecommons.org/licenses/by/4.0/

PY - 2021/11/30

Y1 - 2021/11/30

N2 - Sheaffer blue ink is an effective method to stain arbuscular mycorrhizal (AM) fungi in a variety of plant species. It has, however, received criticism for its potential rapid degradation and short-term viability. The long and medium term storage and viability of stained samples has not, to date, been described for this particular staining method. This short communication reports on the viability of 730 samples stained with Sheaffer® blue ink stored for the duration of four years in microscope slide boxes out of direct sunlight. There was no significant difference in micrograph image quality and presence of stain between years as indicated by the number of AM fungal structures quantified. In conclusion Sheaffer® blue ink stain does not deteriorate in the medium term.

AB - Sheaffer blue ink is an effective method to stain arbuscular mycorrhizal (AM) fungi in a variety of plant species. It has, however, received criticism for its potential rapid degradation and short-term viability. The long and medium term storage and viability of stained samples has not, to date, been described for this particular staining method. This short communication reports on the viability of 730 samples stained with Sheaffer® blue ink stored for the duration of four years in microscope slide boxes out of direct sunlight. There was no significant difference in micrograph image quality and presence of stain between years as indicated by the number of AM fungal structures quantified. In conclusion Sheaffer® blue ink stain does not deteriorate in the medium term.

KW - arbuscular mycorrhizal fungi

KW - blue ink stain

KW - root cortex

KW - storage

KW - degradation

U2 - 10.1099/acmi.0.000292

DO - 10.1099/acmi.0.000292

M3 - Article

VL - 3

JO - Access Microbiology

JF - Access Microbiology

SN - 2516-8290

IS - 11

M1 - 000292

ER -