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Analysis of biofilm production and expression of adhesion structures of circulating Clostridioides difficile strains from Mexico. / Adrian Martinez-Melendez, Adrian; Morfin-Otero, Rayo; Villarreal-Trevino, Licet; Baines, Simon; Camacho-Ortiz, Adrian; Garza-Gonzalez, Elvira.

In: Enfermedades Infecciosas y Microbiologia Clinica, 10.03.2021.

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Adrian Martinez-Melendez, Adrian ; Morfin-Otero, Rayo ; Villarreal-Trevino, Licet ; Baines, Simon ; Camacho-Ortiz, Adrian ; Garza-Gonzalez, Elvira. / Analysis of biofilm production and expression of adhesion structures of circulating Clostridioides difficile strains from Mexico. In: Enfermedades Infecciosas y Microbiologia Clinica. 2021.

Bibtex

@article{17722cd133af482a83ad8cc414becf09,
title = "Analysis of biofilm production and expression of adhesion structures of circulating Clostridioides difficile strains from Mexico",
abstract = "Introduction Clostridioides difficile biofilms are believed to protect the pathogen from antibiotics, in addition to potentially contributing to recurrent infections. Methodology: Biofilm production of 102 C. difficile isolates was determined using the crystal violet staining technique, and detachment assays were performed. The expression levels of cwp84 and slpA genes were evaluated by real-time PCR on selected isolates. Results: More than 70% of isolates (75/102) were strong biofilm producers, and the highest detachment of biofilm was achieved with the proteinase K treatment (>90%). The overall mean expression of cwp84 was higher in RT027 than in RT001 (p = 0.003); among strong biofilm-producing strains, the slpA expression was lower in RT027 than in RT001 (p < 0.000). Conclusions: Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level. Methodology Biofilm production of 102 C. difficile isolates was determined using the crystal violet staining technique, and detachment assays were performed. The expression levels of cwp84 and slpA genes were evaluated by real-time PCR on selected isolates. Results More than 70% of isolates (75/102) were strong biofilm producers, and the highest detachment of biofilm was achieved with the proteinase K treatment (>90%). The overall mean expression of cwp84 was higher in RT027 than in RT001 (p = 0.003); among strong biofilm-producing strains, the slpA expression was lower in RT027 than in RT001 (p < 0.000). Conclusions Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level.",
keywords = "Biofilm, Clostridioides difficile, Cwp84, Ribotype 001, Ribotype 027, slpa",
author = "{Adrian Martinez-Melendez}, Adrian and Rayo Morfin-Otero and Licet Villarreal-Trevino and Simon Baines and Adrian Camacho-Ortiz and Elvira Garza-Gonzalez",
note = "{\textcopyright} 2021 Sociedad Espa{\~n}ola de Enfermedades Infecciosas y Microbiolog{\'i}a Cl{\'i}nica. Published by Elsevier Espa{\~n}a, S.L.U. All rights reserved.",
year = "2021",
month = mar,
day = "10",
doi = "10.1016/j.eimc.2021.01.017",
language = "English",
journal = "Enfermedades Infecciosas y Microbiologia Clinica",
issn = "0213-005X",
publisher = "Ediciones Doyma, S.L.",

}

RIS

TY - JOUR

T1 - Analysis of biofilm production and expression of adhesion structures of circulating Clostridioides difficile strains from Mexico

AU - Adrian Martinez-Melendez, Adrian

AU - Morfin-Otero, Rayo

AU - Villarreal-Trevino, Licet

AU - Baines, Simon

AU - Camacho-Ortiz, Adrian

AU - Garza-Gonzalez, Elvira

N1 - © 2021 Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. Published by Elsevier España, S.L.U. All rights reserved.

PY - 2021/3/10

Y1 - 2021/3/10

N2 - Introduction Clostridioides difficile biofilms are believed to protect the pathogen from antibiotics, in addition to potentially contributing to recurrent infections. Methodology: Biofilm production of 102 C. difficile isolates was determined using the crystal violet staining technique, and detachment assays were performed. The expression levels of cwp84 and slpA genes were evaluated by real-time PCR on selected isolates. Results: More than 70% of isolates (75/102) were strong biofilm producers, and the highest detachment of biofilm was achieved with the proteinase K treatment (>90%). The overall mean expression of cwp84 was higher in RT027 than in RT001 (p = 0.003); among strong biofilm-producing strains, the slpA expression was lower in RT027 than in RT001 (p < 0.000). Conclusions: Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level. Methodology Biofilm production of 102 C. difficile isolates was determined using the crystal violet staining technique, and detachment assays were performed. The expression levels of cwp84 and slpA genes were evaluated by real-time PCR on selected isolates. Results More than 70% of isolates (75/102) were strong biofilm producers, and the highest detachment of biofilm was achieved with the proteinase K treatment (>90%). The overall mean expression of cwp84 was higher in RT027 than in RT001 (p = 0.003); among strong biofilm-producing strains, the slpA expression was lower in RT027 than in RT001 (p < 0.000). Conclusions Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level.

AB - Introduction Clostridioides difficile biofilms are believed to protect the pathogen from antibiotics, in addition to potentially contributing to recurrent infections. Methodology: Biofilm production of 102 C. difficile isolates was determined using the crystal violet staining technique, and detachment assays were performed. The expression levels of cwp84 and slpA genes were evaluated by real-time PCR on selected isolates. Results: More than 70% of isolates (75/102) were strong biofilm producers, and the highest detachment of biofilm was achieved with the proteinase K treatment (>90%). The overall mean expression of cwp84 was higher in RT027 than in RT001 (p = 0.003); among strong biofilm-producing strains, the slpA expression was lower in RT027 than in RT001 (p < 0.000). Conclusions: Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level. Methodology Biofilm production of 102 C. difficile isolates was determined using the crystal violet staining technique, and detachment assays were performed. The expression levels of cwp84 and slpA genes were evaluated by real-time PCR on selected isolates. Results More than 70% of isolates (75/102) were strong biofilm producers, and the highest detachment of biofilm was achieved with the proteinase K treatment (>90%). The overall mean expression of cwp84 was higher in RT027 than in RT001 (p = 0.003); among strong biofilm-producing strains, the slpA expression was lower in RT027 than in RT001 (p < 0.000). Conclusions Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level.

KW - Biofilm

KW - Clostridioides difficile

KW - Cwp84

KW - Ribotype 001

KW - Ribotype 027

KW - slpa

UR - http://www.scopus.com/inward/record.url?scp=85103430485&partnerID=8YFLogxK

U2 - 10.1016/j.eimc.2021.01.017

DO - 10.1016/j.eimc.2021.01.017

M3 - Article

JO - Enfermedades Infecciosas y Microbiologia Clinica

JF - Enfermedades Infecciosas y Microbiologia Clinica

SN - 0213-005X

ER -