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Antisense effects of PNAs in bacteria

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Antisense effects of PNAs in bacteria. / Goh, Shan; Stach, Jem; Good, Liam.

In: Methods in Molecular Biology, Vol. 1050, 2014, p. 223-36.

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Goh, Shan ; Stach, Jem ; Good, Liam. / Antisense effects of PNAs in bacteria. In: Methods in Molecular Biology. 2014 ; Vol. 1050. pp. 223-36.

Bibtex

@article{efe0f601c07d403c96a9cab244a4cdf0,
title = "Antisense effects of PNAs in bacteria",
abstract = "Peptide nucleic acids (PNAs) are a class of artificial DNA/RNA analogues that have unique physicochemical properties, which include a high chemical stability, resistance to nucleases and proteases and higher mismatch sensitivity than DNA. PNAs were initially anticipated to be useful for application in antisense and antigene therapies; however, their poor cellular uptake has limited their use for such purposes in the {"}real world{"}. Recently, it has been shown that the addition of metal complexes to these oligonucleotide analogues could open up new avenues for their utilization in various research fields. Such metallo-constructs have shown great promise, for a diverse range of applications, most notably in the biosensing area. In this chapter, we report on the recent synthetic advances towards the preparation of these {"}(multi)-metallic PNAs{"} on the solid phase.",
keywords = "Amino Acid Sequence, Base Sequence, Cell-Penetrating Peptides, Escherichia coli K12, Gene Silencing, Genes, Reporter, Oligonucleotides, Antisense, Peptide Nucleic Acids, Reproducibility of Results, Journal Article",
author = "Shan Goh and Jem Stach and Liam Good",
year = "2014",
doi = "10.1007/978-1-62703-553-8_18",
language = "English",
volume = "1050",
pages = "223--36",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Antisense effects of PNAs in bacteria

AU - Goh, Shan

AU - Stach, Jem

AU - Good, Liam

PY - 2014

Y1 - 2014

N2 - Peptide nucleic acids (PNAs) are a class of artificial DNA/RNA analogues that have unique physicochemical properties, which include a high chemical stability, resistance to nucleases and proteases and higher mismatch sensitivity than DNA. PNAs were initially anticipated to be useful for application in antisense and antigene therapies; however, their poor cellular uptake has limited their use for such purposes in the "real world". Recently, it has been shown that the addition of metal complexes to these oligonucleotide analogues could open up new avenues for their utilization in various research fields. Such metallo-constructs have shown great promise, for a diverse range of applications, most notably in the biosensing area. In this chapter, we report on the recent synthetic advances towards the preparation of these "(multi)-metallic PNAs" on the solid phase.

AB - Peptide nucleic acids (PNAs) are a class of artificial DNA/RNA analogues that have unique physicochemical properties, which include a high chemical stability, resistance to nucleases and proteases and higher mismatch sensitivity than DNA. PNAs were initially anticipated to be useful for application in antisense and antigene therapies; however, their poor cellular uptake has limited their use for such purposes in the "real world". Recently, it has been shown that the addition of metal complexes to these oligonucleotide analogues could open up new avenues for their utilization in various research fields. Such metallo-constructs have shown great promise, for a diverse range of applications, most notably in the biosensing area. In this chapter, we report on the recent synthetic advances towards the preparation of these "(multi)-metallic PNAs" on the solid phase.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cell-Penetrating Peptides

KW - Escherichia coli K12

KW - Gene Silencing

KW - Genes, Reporter

KW - Oligonucleotides, Antisense

KW - Peptide Nucleic Acids

KW - Reproducibility of Results

KW - Journal Article

U2 - 10.1007/978-1-62703-553-8_18

DO - 10.1007/978-1-62703-553-8_18

M3 - Article

C2 - 24297363

VL - 1050

SP - 223

EP - 236

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -