University of Hertfordshire

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Original languageEnglish
Pages (from-to)100-106
Number of pages7
JournalPhytochemical Analysis
Early online date13 Jan 2016
Publication statusPublished - 1 Mar 2016


The rising trend to consume herbal products for the treatment and/or prevention of minor ailments together with their chemical and pharmacological complexity means there is an urgent need to develop new approaches to their quality and stability. This work looks at the application of one-dimensional diffusion-edited ¹H NMR spectroscopy (1D DOSY) and ¹H NMR with suppression of the ethanol and water signals to the characterization of quality and stability markers in multicomponent herbal medicines/food supplements. The experiments were performed with commercial tinctures of Valeriana officinalis L. (valerian), expired and non-expired, as well as its combination with Hummulus lupulus L. (hops), which is one of the most popular blends of relaxant herbs. These techniques did not require purification or evaporation of components for the qualitative analysis of the mixture, but only the addition of D2O and TSP. The best diagnostic signals were found at  7 ppm (H-11, valerenic acid),  4.2 ppm (H-1, hydroxyvalerenic acid) and  1.5-1.8 ppm (methyl groups in prenylated moieties, α-acids/prenylated flavones). This work concludes on the potential value of 1D DOSY ¹H NMR to provide additional assurance of quality in complex natural mixtures.


This is the peer reviewed version of the following article: Jose M. Prieto, Maria Mellinas-Gomez, Mire Zloh, ‘Application of diffusion-edited and solvent suppression 1H-NMR to the direct analysis of markers in valerian-hop liquid herbal products’, Phytochemical Analysis, Vol 27(2): 100-106, first published online January 13, 2016, which has been published in final form at doi: 10.1002/pca.2603 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving Copyright © 2016 John Wiley & Sons, Ltd.

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