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Biological evaluations of novel 2,3,3-Trisphosphonate in osteoclastic and osteoblastic activities

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Biological evaluations of novel 2,3,3-Trisphosphonate in osteoclastic and osteoblastic activities. / Cheong, Yuen Ki; Huang, Mei; Detsch, Rainer ; Boccaccini, Aldo R.; Ren, Guogang.

In: General Medicine Open, Vol. 2, No. 1, 08.12.2017, p. 1-10.

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@article{a2f45c0c393a4d42be16332899a3a733,
title = "Biological evaluations of novel 2,3,3-Trisphosphonate in osteoclastic and osteoblastic activities",
abstract = "Bisphosphonates (BPs) are the first line treatment for many bone diseases including hypercalcimia associated with bone malignancies. In this paper, we introduce a new analogue of bisphosphonate called the 2,3,3-Trisphosphonate (2,3,3-TriPP) that was synthesised in a two steps reaction. In vitro investigations using a medically known bisphosphonate (Etidronate) and the 2,3,3-TrisPP were performed with an aim to evaluate biological effect of this novel compound in major bone cells. 2,3,3-TrisPP showed to have potential to supress the bone resorption process, as our data found that this novel compound exhibited cytotoxic effect in osteoclastic cells at a low concentration of 0.172 mg/mL (LC50). A molecular docking computational simulation calculated a high level of binding affinity between the human farnesyl pyrophosphate synthase (hFPPS) and 2,3,3-TrisPP. This calculation suggested 2,3,3TrisPP may have undergone the mevalonate pathway to prevent the prenylation step during biosynthesis and subsequently resulted in the deactivation of osteoclastic cells. Finally, high levels of osteoblast mineralisation potentials were recorded upon treatments with 2,3,3-TrisPP (0.01-0.1 mg/ml), which implied 2,3,3-TrsiPP may also facilitate bone regeneration.",
author = "Cheong, {Yuen Ki} and Mei Huang and Rainer Detsch and Boccaccini, {Aldo R.} and Guogang Ren",
year = "2017",
month = dec,
day = "8",
doi = "10.15761/GMO.1000121",
language = "English",
volume = "2",
pages = "1--10",
journal = "General Medicine Open",
issn = "2515-4737",
number = "1",

}

RIS

TY - JOUR

T1 - Biological evaluations of novel 2,3,3-Trisphosphonate in osteoclastic and osteoblastic activities

AU - Cheong, Yuen Ki

AU - Huang, Mei

AU - Detsch, Rainer

AU - Boccaccini, Aldo R.

AU - Ren, Guogang

PY - 2017/12/8

Y1 - 2017/12/8

N2 - Bisphosphonates (BPs) are the first line treatment for many bone diseases including hypercalcimia associated with bone malignancies. In this paper, we introduce a new analogue of bisphosphonate called the 2,3,3-Trisphosphonate (2,3,3-TriPP) that was synthesised in a two steps reaction. In vitro investigations using a medically known bisphosphonate (Etidronate) and the 2,3,3-TrisPP were performed with an aim to evaluate biological effect of this novel compound in major bone cells. 2,3,3-TrisPP showed to have potential to supress the bone resorption process, as our data found that this novel compound exhibited cytotoxic effect in osteoclastic cells at a low concentration of 0.172 mg/mL (LC50). A molecular docking computational simulation calculated a high level of binding affinity between the human farnesyl pyrophosphate synthase (hFPPS) and 2,3,3-TrisPP. This calculation suggested 2,3,3TrisPP may have undergone the mevalonate pathway to prevent the prenylation step during biosynthesis and subsequently resulted in the deactivation of osteoclastic cells. Finally, high levels of osteoblast mineralisation potentials were recorded upon treatments with 2,3,3-TrisPP (0.01-0.1 mg/ml), which implied 2,3,3-TrsiPP may also facilitate bone regeneration.

AB - Bisphosphonates (BPs) are the first line treatment for many bone diseases including hypercalcimia associated with bone malignancies. In this paper, we introduce a new analogue of bisphosphonate called the 2,3,3-Trisphosphonate (2,3,3-TriPP) that was synthesised in a two steps reaction. In vitro investigations using a medically known bisphosphonate (Etidronate) and the 2,3,3-TrisPP were performed with an aim to evaluate biological effect of this novel compound in major bone cells. 2,3,3-TrisPP showed to have potential to supress the bone resorption process, as our data found that this novel compound exhibited cytotoxic effect in osteoclastic cells at a low concentration of 0.172 mg/mL (LC50). A molecular docking computational simulation calculated a high level of binding affinity between the human farnesyl pyrophosphate synthase (hFPPS) and 2,3,3-TrisPP. This calculation suggested 2,3,3TrisPP may have undergone the mevalonate pathway to prevent the prenylation step during biosynthesis and subsequently resulted in the deactivation of osteoclastic cells. Finally, high levels of osteoblast mineralisation potentials were recorded upon treatments with 2,3,3-TrisPP (0.01-0.1 mg/ml), which implied 2,3,3-TrsiPP may also facilitate bone regeneration.

U2 - 10.15761/GMO.1000121

DO - 10.15761/GMO.1000121

M3 - Article

VL - 2

SP - 1

EP - 10

JO - General Medicine Open

JF - General Medicine Open

SN - 2515-4737

IS - 1

ER -