University of Hertfordshire

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Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters. / Shah, Niksha; Naseby, David.

In: Journal of Applied Microbiology, Vol. 117, No. 5, 11.2014, p. 1373-1387.

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@article{27813199074e493f9c99086b535c555d,
title = "Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters",
abstract = "Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux",
author = "Niksha Shah and David Naseby",
year = "2014",
month = nov,
doi = "10.1111/jam.12635",
language = "English",
volume = "117",
pages = "1373--1387",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters

AU - Shah, Niksha

AU - Naseby, David

PY - 2014/11

Y1 - 2014/11

N2 - Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux

AB - Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs allows real-time monitoring, rapid analysis and high-throughput screening of products. Microbial growth can be studied by measuring constitutive gene expression. The aim is to develop whole-cell microbial biosensors with Pseudomonas aeruginosa and quantify their growth rate by measuring constitutive expression of lux

U2 - 10.1111/jam.12635

DO - 10.1111/jam.12635

M3 - Article

VL - 117

SP - 1373

EP - 1387

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

ER -