University of Hertfordshire

Characterisation of microvesicles released from cells constitutively and upon stimulation

Research output: Contribution to conferencePosterpeer-review


  • SA133P009

    Accepted author manuscript, 102 KB, PDF document

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Original languageEnglish
Publication statusPublished - 13 Sep 2012
EventMicrovesiculation and Disease, a Biochemical Society Focused Meeting: Microvesiculation and Disease - London Metropolitan University, London, United Kingdom
Duration: 13 Sep 201214 Sep 2012


ConferenceMicrovesiculation and Disease, a Biochemical Society Focused Meeting
Country/TerritoryUnited Kingdom
Internet address


Constitutively released microvesicles (cMVs) are released as a part of normal cell physiology and this is summarised in Fig. 1 (1). However stimulated microvesicles (sMVs) are released as a result of a number of possible stress factors (2, 3). We found sMVs to be released in higher numbers than cMVs, typically ten-fold higher numbers, in the same time frame, and where the stress factor was a pharmacological agent, the microvesiculation was an attempt to release this chemical stress factor. Using a mass sensing technique, the sMVs were released over a 15 min period after stimulation. Using sizing beads on a flow cytometer and by transmission electron microscopy the cMVs were typically smaller (less than 300 nm in diameter) than sMVs (300-500 nm in diameter). However cMVs were found to carry more protein. By contrast, phosphatidylserine expression was greater on the larger sMVs, which also more effectively inhibited complement-mediated lysis.

ID: 13382516