University of Hertfordshire

By the same authors

Characterization of microvesicles released from cells constitutively and upon stimulation

Research output: Contribution to conferenceAbstractpeer-review


  • Dan Stratton
  • Samuel Antwi-Baffour
  • Gareth Williams
  • Sigrun Lange
  • Jameel Inal
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Original languageEnglish
Number of pages1
Publication statusPublished - 15 Apr 2012
EventInternational Society for Extracellular Vesicles 1st annual meeting: ISEV meeting, 2012, Gothenburg - University of Gothenburg, Gothenburg, Sweden
Duration: 18 Apr 201221 Apr 2012


ConferenceInternational Society for Extracellular Vesicles 1st annual meeting
Abbreviated titleISEV, 2012
Internet address


Constitutively released microvesicles (cMVs) are released as a part of
normal cell physiology. However, stimulated microvesicles (sMVs) are
released as a result of a number of possible stress factors. We found
sMVs to be released in higher numbers than cMVs, typically 10-fold
higher numbers, in the same time frame, and where the stress factor
was a pharmacological agent, the microvesiculation was an attempt
to release this chemical stress factor. Using a mass sensing
technique, the sMVs were released over a 15-minutes period after
stimulation. Using sizing beads on a flow cytometer and by
transmission electron microscopy, the cMVs were typically smaller
(less than 300 nm in diameter) than sMVs (300500 nm in diameter).
However, cMVs were found to carry more protein. By contrast,
phosphatidylserine expression was greater on the larger sMVs, which
also more effectively inhibited complement-mediated lysis.

ID: 13397205