University of Hertfordshire

By the same authors

Contact time effects of ketamine and related novel psychoactive substances on rat bladder

Research output: Contribution to conferencePosterpeer-review

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Contact time effects of ketamine and related novel psychoactive substances on rat bladder. / Gant, Alex; Kjellin, Patricia; Fergus, Suzanne; Benham, Christopher; Lione, Lisa.

2017. Poster session presented at V International Conference on Novel Psychoactive Substances, Vienna, Austria.

Research output: Contribution to conferencePosterpeer-review

Harvard

Gant, A, Kjellin, P, Fergus, S, Benham, C & Lione, L 2017, 'Contact time effects of ketamine and related novel psychoactive substances on rat bladder', V International Conference on Novel Psychoactive Substances, Vienna, Austria, 23/10/17 - 24/10/17.

APA

Gant, A., Kjellin, P., Fergus, S., Benham, C., & Lione, L. (2017). Contact time effects of ketamine and related novel psychoactive substances on rat bladder. Poster session presented at V International Conference on Novel Psychoactive Substances, Vienna, Austria.

Vancouver

Gant A, Kjellin P, Fergus S, Benham C, Lione L. Contact time effects of ketamine and related novel psychoactive substances on rat bladder. 2017. Poster session presented at V International Conference on Novel Psychoactive Substances, Vienna, Austria.

Author

Gant, Alex ; Kjellin, Patricia ; Fergus, Suzanne ; Benham, Christopher ; Lione, Lisa. / Contact time effects of ketamine and related novel psychoactive substances on rat bladder. Poster session presented at V International Conference on Novel Psychoactive Substances, Vienna, Austria.

Bibtex

@conference{bdf27d849e9a431fbba50f477755aa2c,
title = "Contact time effects of ketamine and related novel psychoactive substances on rat bladder",
abstract = "Introduction: Due partly to their dissociative properties, ketamine and related new psychoactive substances (NPS) are widely used as recreational drugs. This has led to the discovery of a link between chronic use of ketamine and methoxetamine and the development of cystitis, characterized by chronic inflammation and urothelial ulceration. Recent findings indicate that ketamine has a direct toxic effect on human urothelial cells causing thinning of the urothelium within 3 days (1). We have developed a rat organ culture assay incubating ketamine or related NPS with rat bladder tissue, for up to 10 days, to establish whether NPS exhibit direct contact time effects. Methods: Rat bladder was dissected in half longitudinally and cultured at 37°C with 3mM ketamine or related NPS (methoxetamine, diphenidine, methoxphenidine) for 1, 3, 5 or 10 days. One half of each bladder was incubated with culture media (no drug) as time matched control. Bladders were then fixed in 10% formalin for 24 hours, dehydrated through graded alcohols and cleared with xylene, embedded in paraffin wax, sectioned at 5uM thickness and stained for visualisation of nuclei, cytoplasm, tight junctions and nitric oxide synthase. Results: Preliminary data indicates that after 3 days culture with ketamine there is thinning of rat bladder urothelium, loss of connective tissue and thickening of detrusor muscle. The effects of ketamine, methoxetamine, diphenidine and methoxphenidine on the integrity of urothelium cells, tight junctions and detrusor muscle will be presented.Conclusions: These results will provide insight into the long term effects of ketamine and NPS in the bladder, for which detailed information is lacking.(1) Baker et al., 2016 Ketamine-Induced Apoptosis in Normal Human Urothelial Cells Am J Pathology, 186 (5) 1268. ",
author = "Alex Gant and Patricia Kjellin and Suzanne Fergus and Christopher Benham and Lisa Lione",
year = "2017",
month = oct,
day = "23",
language = "English",
note = "V International Conference on Novel Psychoactive Substances ; Conference date: 23-10-2017 Through 24-10-2017",
url = "https://www.unodc.org/LSS/Announcement/Details/9426f9ab-77ce-4845-8008-0121feed947b",

}

RIS

TY - CONF

T1 - Contact time effects of ketamine and related novel psychoactive substances on rat bladder

AU - Gant, Alex

AU - Kjellin, Patricia

AU - Fergus, Suzanne

AU - Benham, Christopher

AU - Lione, Lisa

PY - 2017/10/23

Y1 - 2017/10/23

N2 - Introduction: Due partly to their dissociative properties, ketamine and related new psychoactive substances (NPS) are widely used as recreational drugs. This has led to the discovery of a link between chronic use of ketamine and methoxetamine and the development of cystitis, characterized by chronic inflammation and urothelial ulceration. Recent findings indicate that ketamine has a direct toxic effect on human urothelial cells causing thinning of the urothelium within 3 days (1). We have developed a rat organ culture assay incubating ketamine or related NPS with rat bladder tissue, for up to 10 days, to establish whether NPS exhibit direct contact time effects. Methods: Rat bladder was dissected in half longitudinally and cultured at 37°C with 3mM ketamine or related NPS (methoxetamine, diphenidine, methoxphenidine) for 1, 3, 5 or 10 days. One half of each bladder was incubated with culture media (no drug) as time matched control. Bladders were then fixed in 10% formalin for 24 hours, dehydrated through graded alcohols and cleared with xylene, embedded in paraffin wax, sectioned at 5uM thickness and stained for visualisation of nuclei, cytoplasm, tight junctions and nitric oxide synthase. Results: Preliminary data indicates that after 3 days culture with ketamine there is thinning of rat bladder urothelium, loss of connective tissue and thickening of detrusor muscle. The effects of ketamine, methoxetamine, diphenidine and methoxphenidine on the integrity of urothelium cells, tight junctions and detrusor muscle will be presented.Conclusions: These results will provide insight into the long term effects of ketamine and NPS in the bladder, for which detailed information is lacking.(1) Baker et al., 2016 Ketamine-Induced Apoptosis in Normal Human Urothelial Cells Am J Pathology, 186 (5) 1268.

AB - Introduction: Due partly to their dissociative properties, ketamine and related new psychoactive substances (NPS) are widely used as recreational drugs. This has led to the discovery of a link between chronic use of ketamine and methoxetamine and the development of cystitis, characterized by chronic inflammation and urothelial ulceration. Recent findings indicate that ketamine has a direct toxic effect on human urothelial cells causing thinning of the urothelium within 3 days (1). We have developed a rat organ culture assay incubating ketamine or related NPS with rat bladder tissue, for up to 10 days, to establish whether NPS exhibit direct contact time effects. Methods: Rat bladder was dissected in half longitudinally and cultured at 37°C with 3mM ketamine or related NPS (methoxetamine, diphenidine, methoxphenidine) for 1, 3, 5 or 10 days. One half of each bladder was incubated with culture media (no drug) as time matched control. Bladders were then fixed in 10% formalin for 24 hours, dehydrated through graded alcohols and cleared with xylene, embedded in paraffin wax, sectioned at 5uM thickness and stained for visualisation of nuclei, cytoplasm, tight junctions and nitric oxide synthase. Results: Preliminary data indicates that after 3 days culture with ketamine there is thinning of rat bladder urothelium, loss of connective tissue and thickening of detrusor muscle. The effects of ketamine, methoxetamine, diphenidine and methoxphenidine on the integrity of urothelium cells, tight junctions and detrusor muscle will be presented.Conclusions: These results will provide insight into the long term effects of ketamine and NPS in the bladder, for which detailed information is lacking.(1) Baker et al., 2016 Ketamine-Induced Apoptosis in Normal Human Urothelial Cells Am J Pathology, 186 (5) 1268.

M3 - Poster

T2 - V International Conference on Novel Psychoactive Substances

Y2 - 23 October 2017 through 24 October 2017

ER -