University of Hertfordshire

Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle

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Documents

  • Shan Goh
  • Jeannine Kolakowski
  • Angela Holder
  • Mark Pfuhl
  • Daniel Ngugi
  • Keith Ballingall
  • Kata Tombacz
  • Dirk Werling
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Original languageEnglish
Article number674484
Number of pages18
JournalFrontiers in Immunology
Volume12
IssueJuly 2021
DOIs
Publication statusPublished - 8 Jul 2021

Abstract

East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention relies on the so called “Infection and Treatment Method” (ITM), which is costly, complex, laborious, difficult to standardise on a commercial scale and results in a parasite strain-specific, MHC class Irestricted cytotoxic T cell response. We therefore attempted to develop a safe, affordable, stable, orally applicable and potent subunit vaccine for ECF using five different T. parva schizont antigens (Tp1, Tp2, Tp9, Tp10 and N36) and Saccharomyces cerevisiae as an expression platform. Full-length Tp2 and Tp9 as well as fragments of Tp1 were successfully expressed on the surface of S. cerevisiae. In vitro analyses highlighted that recombinant yeast expressing Tp2 can elicit IFNg responses using PBMCs from ITM immunized calves, while Tp2 and Tp9 induced IFNg responses from enriched bovine CD8+ T cells. A subsequent in vivo study showed that oral administration of heat inactivated, freeze-dried yeast stably expressing Tp2 increased total murine serum IgG over time, but more importantly, induced Tp2-specific serum IgG antibodies in individual mice compared to the control group. While these results will require subsequent experiments to verify induction of protection in neonatal calves, our data indicates that oral application of yeast expressing Theileria antigens could provide an affordable and easy vaccination platform for sub-Saharan Africa. Evaluation of antigen-specific cellular
immune responses, especially cytotoxic CD8+ T cell immunity in cattle will further
contribute to the development of a yeast-based vaccine for ECF.

Notes

© 2021 Goh, Kolakowski, Holder, Pfuhl, Ngugi, Ballingall, Tombacz and Werling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/

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