University of Hertfordshire

Dysregulated placental microRNAs in Early and Late onset Preeclampsia

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  • Placenta

    Accepted author manuscript, 571 KB, PDF document

  • Alexandra Lykoudi
  • Aggeliki Kolialexi
  • George I Lambrou
  • Maria Braoudaki
  • Charalampos Siristatidis
  • George Konstantinos Papaioanou
  • Maria Tzetis
  • Ariadni Mavrou
  • Nikolas Papantoniou
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Original languageEnglish
Pages (from-to)24-32
Number of pages9
JournalPlacenta
Volume61
Early online date14 Nov 2017
DOIs
Publication statusPublished - 1 Jan 2018

Abstract

INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies.

METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction.

RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR.

DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.

ID: 14882038