University of Hertfordshire

  • Yanning Ding
  • William J. Brackenbury
  • Pinar U. Onganer
  • Ximena Montano
  • Louise M. Porter
  • Lucy F. Bates
  • Mustafa B.A. Djamgoz
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Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalJournal of Cellular Physiology
Volume215
Issue1
Early online date24 Oct 2007
DOIs
Publication statusPublished - Apr 2008

Abstract

The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity

ID: 952152